Benedict C R, Mathew B, Rex K A, Cartwright J, Sordahl L A
Circ Res. 1986 Jan;58(1):58-67. doi: 10.1161/01.res.58.1.58.
The role of platelets in contributing to occlusive coronary artery thrombus formation remains unresolved. A large number of studies have utilized in vitro techniques to study platelet aggregation. This report describes a model of spontaneous in vivo thrombus formation which involves application of current in the left circumflex coronary artery of the dog. Changes in mean coronary blood flow velocity (50% above control) are used to predict the point at which current can be discontinued without interrupting the ongoing process of thrombus formation. Thrombus formation proceeds to total vessel occlusion within 62 +/- 18 minutes after discontinuation of current. Coronary sinus plasma serotonin concentrations are used as an in vivo index of platelet aggregation during thrombus formation. Plasma serotonin levels increased only slightly above baseline levels during initial thrombus formation. Coronary sinus serotonin levels rose markedly after cessation of current, reaching a peak just prior to total vessel occlusion. The marked increase in serotonin concentration observed in the latter stages of thrombus formation strongly suggests that platelet aggregation is a significant factor in the evolution of an occlusive coronary thrombus.
血小板在导致冠状动脉闭塞性血栓形成过程中的作用仍未明确。大量研究利用体外技术来研究血小板聚集。本报告描述了一种体内自发性血栓形成模型，该模型涉及在犬的左旋冠状动脉中通电流。平均冠状动脉血流速度的变化（比对照高50%）用于预测在不中断正在进行的血栓形成过程的情况下可以停止电流的时间点。在停止电流后62±18分钟内，血栓形成进展至血管完全闭塞。冠状窦血浆5-羟色胺浓度用作血栓形成过程中血小板聚集的体内指标。在初始血栓形成期间，血浆5-羟色胺水平仅比基线水平略有升高。电流停止后，冠状窦5-羟色胺水平显著上升，在血管完全闭塞前达到峰值。在血栓形成后期观察到的5-羟色胺浓度的显著增加强烈表明血小板聚集是冠状动脉闭塞性血栓演变的一个重要因素。
