LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
CESAM, Department of Biology, University of Aveiro, 3810-193 Aveiro, Portugal.
ACS Appl Bio Mater. 2024 Nov 18;7(11):7748-7757. doi: 10.1021/acsabm.4c01368. Epub 2024 Oct 21.
Antimicrobial resistance remains an enduring global health issue, manifested when microorganisms, such as bacteria, lack responsiveness to antimicrobial treatments. Photodynamic inactivation (PDI) of microorganisms arises as a noninvasive, nontoxic, and repeatable alternative for the inactivation of a broad range of pathogens. So, this study reports the synthesis, structural characterization, and photophysical properties of a new tetra-β-substituted pyridinium-pyrazolyl zinc(II) phthalocyanine (ZnPc ) that was compared with two previously described pyridinium-pyrazolyl ZnPcs and . The PDI efficacy of these three ZnPcs (-) against a drug-resistant Gram-positive bacterium (as ) and a Gram-negative bacterium (as ) is also reported. The PDI efficacy toward these bacteria was examined with ZnPcs - in the 5.0-10.0 μM range using a white light source with an irradiance of 150 mW/cm. All ZnPcs displayed a significant PDI activity against , with reductions superior to 3 Log CFU/mL. Increasing the treatment time, the was inactivated until the detection limit of the method (>6.3 Log CFU/mL) using the quaternized ZnPcs - (10.0 μM, 120 min) being the inactivation time was reduced when added the KI for ZnPcs and . These findings demonstrate the effective PDI performance of pyridinium-pyrazolyl group-bearing PSs, indicating their potential use as a versatile antimicrobial agent for managing infections induced by Gram-negative and Gram-positive bacteria.
抗微生物药物耐药性仍然是一个持久存在的全球卫生问题，当微生物（如细菌）对抗微生物治疗缺乏反应时，就会出现这种情况。光动力失活（PDI）作为一种非侵入性、无毒和可重复的替代方法，可用于失活广泛的病原体。因此，本研究报告了一种新的四-β取代吡啶基-吡唑基锌（II）酞菁（ZnPc）的合成、结构表征和光物理性质，该化合物与之前描述的两种吡啶基-吡唑基 ZnPcs和进行了比较。还报告了这三种 ZnPcs（-）对一种耐药性革兰氏阳性菌（作为）和一种革兰氏阴性菌（作为）的 PDI 功效。使用辐照度为 150 mW/cm 的白光光源，在 5.0-10.0 μM 的范围内，用 ZnPcs-检查了这些细菌的 PDI 功效。所有 ZnPcs 对都表现出显著的 PDI 活性，减少量超过 3 Log CFU/mL。随着处理时间的增加，使用季铵化 ZnPcs-（10.0 μM，120 分钟）直至方法检测限（>6.3 Log CFU/mL）失活，当添加 KI 时，ZnPcs 和的失活时间减少。这些发现证明了带有吡啶基-吡唑基基团的 PSs 的有效 PDI 性能，表明它们具有作为革兰氏阴性和革兰氏阳性细菌感染的多功能抗菌剂的潜在用途。
