Clementi Romina, Vargas Maria Angela, Cid Mariana, Salvatierra Nancy, Comín Romina, Tempesti Tomas
Departamento de Química Orgánica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Córdoba, X5000HUA, Argentina.
Laboratorio de Microbiología, Hospital Provincial Florencio Diaz, Córdoba, Argentina.
Macromol Biosci. 2025 Jan;25(1):e2400334. doi: 10.1002/mabi.202400334. Epub 2024 Oct 29.
In this study, the fabrication and characterization of Zn-phthalocyanine/gelatin nanofibrous membranes is reported using the electrospinning technique. The membranes exhibit a homogeneous distribution of Zn-phthalocyanine within the gelatin matrix, maintaining the structural integrity and photosensitizing properties of the phthalocyanine. Scanning electron microscopy revealed that the electrospun fibers possess diameters ranging results as 100-300, 200-700, and 300-800 nm for Gel, ZnPc/Gel 1, and ZnPc/Gel 2, respectively. The addition of ZnPc does not decrease the hydrophilicity of the Gel membrane. The nanofibrous membranes showed good cytocompatibility, as indicated by the high viability of Vero cells exposed to membrane extracts. Furthermore, these composites supported cell adhesion and proliferation on their surfaces. The two Zn-phthalocyanine/gelatin nanofiber formulations exhibited significant antimicrobial activity toward Escherichia Coli (E. Coli) and Staphylococcus Aureus (S. Aureus) under visible light illumination, achieving reductions of 3.4 log and 3.6 log CFU mL for E. coli, and 3.9 log and 4.1 log CFU mL for S. aureus. These results demonstrate the potential of Zn-phthalocyanine/gelatin nanofibrous membranes as effective agents in antibacterial photodynamic therapy, providing a promising solution to control bacterial infections and antibiotic resistance.
在本研究中，报道了采用静电纺丝技术制备锌酞菁/明胶纳米纤维膜及其表征。这些膜在明胶基质中呈现出锌酞菁的均匀分布，保持了酞菁的结构完整性和光敏特性。扫描电子显微镜显示，对于明胶（Gel）、锌酞菁/明胶1（ZnPc/Gel 1）和锌酞菁/明胶2（ZnPc/Gel 2），静电纺丝纤维的直径分别为100 - 300纳米、200 - 700纳米和300 - 800纳米。添加锌酞菁不会降低明胶膜的亲水性。纳米纤维膜表现出良好的细胞相容性，这通过暴露于膜提取物的Vero细胞的高活力得以体现。此外，这些复合材料支持细胞在其表面的黏附和增殖。两种锌酞菁/明胶纳米纤维配方在可见光照射下对大肠杆菌（E. Coli）和金黄色葡萄球菌（S. Aureus）表现出显著的抗菌活性，对大肠杆菌的菌落形成单位（CFU）减少了3.4个对数和3.6个对数每毫升，对金黄色葡萄球菌减少了3.9个对数和4.1个对数每毫升。这些结果证明了锌酞菁/明胶纳米纤维膜作为抗菌光动力疗法中有效药剂的潜力，为控制细菌感染和抗生素耐药性提供了一个有前景的解决方案。
