Dual cryoprotection of gelatin-tea polyphenol microgels on surimi by targeting for ice inhibition and component stabilization.

In this study, the gelatine-polyphenol microgels with dual cryoprotective roles were constructed by regulating the ratio of gelatin to tea polyphenols (1:50-1:250). The physicochemical attributes, ice recrystallization inhibition ability of microgels, and their dosage effects (1 %, 2.5 % and 4 %, w/w) on surimi were investigated. The results indicated that increased gelatin caused the reduced size and enhanced viscosity of microgels. Except for high viscosity and antioxidant activity, the GP-5 group also showed great IRI ability with minimum size distribution (125-214 μm) of ice crystals. Furthermore, 2.5 G group and S group had a comparable TVB-N (3.81, 4.34 mg/100 g), TBARS (1.18, 1.32 mg/kg), sulfhydryl contents (29.52, 25.48 μmol/g) and Ca-ATPase activity (0.44, 0.36 μmolPi/gprot/h). Compared to uneven free water distribution of control group, S and 2.5 G group show more even immobilized-water distribution. Thereafter, the dual cryoprotective functions of microgels in surimi offer valuable insights for the development of effective antifreeze agents.