Spychała J, Marszałek J
Comp Biochem Physiol B. 1986;83(1):169-71. doi: 10.1016/0305-0491(86)90348-2.
Two molecular forms of AMP deaminase have been revealed by phosphocellulose column chromatography in the liver of uricotelic lizard. The calculated S0.5 value of the purified lizard liver AMP deaminase was 2.5 +/- 0.1 for the form I and 3.6 +/- 0.4 for the form II. Both forms of the enzyme were activated by ATP and ADP but the form II to a much higher extent. GTP activated only the form II and inorganic phosphate inhibited both forms. The occurrence of multiple forms of liver AMP deaminase in uricotelic species, as well as its difference from the mammalian enzyme regulation by GTP is suggested to be connected with the uricotelism in these animals.
通过磷酸纤维素柱色谱法，在尿酸排泄型蜥蜴的肝脏中发现了两种分子形式的AMP脱氨酶。纯化后的蜥蜴肝脏AMP脱氨酶I型的计算S0.5值为2.5±0.1，II型为3.6±0.4。两种形式的酶均被ATP和ADP激活，但II型的激活程度更高。GTP仅激活II型，无机磷酸盐则抑制两种形式的酶。尿酸排泄型物种肝脏中存在多种形式的AMP脱氨酶，以及其与哺乳动物GTP酶调节的差异，被认为与这些动物的尿酸排泄有关。
