Machado-Junior Pedro Alves, Dias Marcelo Santiago Soares, de Souza Ana Beatriz Farias, Lopes Leonardo Spinelli Estevão, Menezes Tatiana Prata, Talvani André, Brochard Laurent, Bezerra Frank Silva
Laboratory of Experimental Pathophysiology (LAFEx), Department of Biological Sciences and Center of Research in Biological Sciences, Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil.
Laboratory of Immunobiology of Inflammation (LABIIN), Department of Biological Sciences (DECBI), Institute of Exact and Biological Sciences (ICEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35400-000, Brazil.
Respir Physiol Neurobiol. 2025 Jan;331:104361. doi: 10.1016/j.resp.2024.104361. Epub 2024 Oct 19.
Mechanical ventilation (MV) is a life support method used to treat patients with respiratory failure. High tidal volumes during MV can cause ventilator-induced lung injury (VILI), but also affect other organs, such as the diaphragm (Dia) causing ventilator-induced diaphragmatic dysfunction (VIDD). VIDD is often associated with a complicated course on MV. Sepsis can induce inflammation and oxidative stress, contributing to the impairment of the Dia and worsening of the prognosis. This study evaluated the additive or synergistic effects of a short course of mechanical ventilation on Dia in healthy and septic adult mice.
32 adult male C57BL/6 mice were randomly into four groups: Control (CG), non-ventilated animals instilled with saline solution (PBS1x); Lipopolysaccharide (LPS), non-ventilated animals instilled with PBS solution containing lipopolysaccharide; Mechanical Ventilation (MV) for 1 h, ventilated animals instilled with PBS solution; and Mechanical Ventilation and LPS (MV+LPS), ventilated animals instilled with PBS solution containing LPS. At the end of the experimental protocol, the animals were euthanized, then blood and diaphragm tissue samples were collected.
Evaluation of leukocyte/blood parameters and diaphragm muscle showed that MV, LPS and the combination of both were able to increase neutrophil count, creatine kinase, inflammatory mediators and oxidative stress in all groups compared to the control. MV and sepsis combined had additive effects on inflammation and lipid peroxidation.
A short course of Mechanical ventilation promotes inflammation and oxidative stress and, its combination with sepsis further increases local and systemic inflammation.
机械通气（MV）是用于治疗呼吸衰竭患者的一种生命支持方法。MV期间高潮气量可导致呼吸机诱导的肺损伤（VILI），还会影响其他器官，如膈肌（Dia），导致呼吸机诱导的膈肌功能障碍（VIDD）。VIDD常与MV过程复杂相关。脓毒症可诱导炎症和氧化应激，导致膈肌功能受损并使预后恶化。本研究评估了短期机械通气对健康和脓毒症成年小鼠膈肌的叠加或协同作用。
32只成年雄性C57BL/6小鼠随机分为四组：对照组（CG），即经鼻滴注生理盐水（PBS1x）的未通气动物；脂多糖组（LPS），即经鼻滴注含脂多糖的PBS溶液的未通气动物；机械通气1小时组（MV），即经鼻滴注PBS溶液的通气动物；机械通气加脂多糖组（MV+LPS），即经鼻滴注含脂多糖的PBS溶液的通气动物。实验方案结束时，对动物实施安乐死，然后采集血液和膈肌组织样本。
白细胞/血液参数和膈肌肌肉评估显示，与对照组相比，MV、LPS以及两者联合均能增加所有组的中性粒细胞计数、肌酸激酶、炎症介质和氧化应激。MV与脓毒症联合对炎症和脂质过氧化具有叠加作用。
短期机械通气可促进炎症和氧化应激，其与脓毒症联合可进一步加重局部和全身炎症。
