Marine Research Institute, Klaipėda University, Universiteto av. 17, LT-92294, Klaipeda, Lithuania.
Departament of Marine Biology and Biotechnology, Faculty of Oceanography and Geography, University of Gdańsk, Marszałka Piłsudskiego 46, 81-378, Gdynia, Poland.
Sci Rep. 2024 Oct 21;14(1):24686. doi: 10.1038/s41598-024-76064-y.
Polyphasic approach has become a generally accepted method for the classification of cyanobacteria. In this study, we present a detailed characterisation of two strains, KUCC C1 and KUCC C2, isolated from the Curonian Lagoon and classified to the Aphanizomenon genus. Despite phylogenetic similarity, the strains differ with respect to morphology, ultrastructure characteristics, and the metabolite profile. In the KUCC C1 extract, three unknown peptides and eight anabaenopeptins were detected, while KUCC C2 produced one unknown peptide and one aeruginosin. In both strains, a total of eleven pigments were detected. The production of myxoxantophyll, chlorophyll-a, chlorophylide-a, and zeaxanthin was higher in KUCC C2 than in KUCC C1. Extracts from both strains of Aphanizomenon also had different effects in antibacterial, anticancer and enzyme inhibition assays. Comprehensive analyses of Aphanizomenon strains performed in this study showed significant diversity between the isolates from the same bloom sample. These differences should be considered when exploring the ecological significance and biotechnological potential of a given population.
多相分类法已成为蓝藻分类的一种通用方法。在这项研究中，我们详细描述了从库尔斯沙嘴泻湖分离到的两个菌株KUCC C1 和 KUCC C2，它们被分类到鱼腥藻属。尽管在系统发育上具有相似性，但这两个菌株在形态、超微结构特征和代谢产物谱方面存在差异。在 KUCC C1 提取物中，检测到三种未知肽和八种鱼腥藻肽，而 KUCC C2 则产生一种未知肽和一种鱼腥藻素。在这两个菌株中，共检测到 11 种色素。KUCC C2 中 myxoxanthophyll、叶绿素-a、叶绿素-a 和玉米黄质的产量高于 KUCC C1。鱼腥藻的提取物在抗菌、抗癌和抑制酶活性的测定中也有不同的效果。本研究对鱼腥藻菌株的综合分析表明，来自同一水华样本的分离株之间存在显著的多样性。在探索特定种群的生态意义和生物技术潜力时，应该考虑到这些差异。
