[新型冠状病毒感染且肺组织中病毒蛋白持续存在的肺腺癌患者中的ALDH1、CD133、CD34阳性癌症干细胞]
[ALDH1, CD133, CD34-positive cancer stem cells in lung adenocarcinoma in patients who had a new coronavirus infection and retained the persistence of viral proteins in the lung tissue].
作者信息
Kogan E A, Shchelokova E E, Demura T A, Zharkov N V, Kichigina O N, Kovyazina N V, Mordovina A I, Zelenchenkova P I, Meerovich G A, Reshetov I V
机构信息
I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia.
Russian University of Medicine, Moscow, Russia.
出版信息
Arkh Patol. 2024;86(5):5-14. doi: 10.17116/patol2024860515.
UNLABELLED
Lung cancer occupies a leading position in the structure of global cancer morbidity and mortality, due to the biological properties of the key pool of tumor cells - cancer stem cells (CSCs). The effects of SARS-CoV2 on tumor CSCs and its niche have not been studied.
OBJECTIVE
To study CSCs in lung adenocarcinomas after COVID19.
MATERIAL AND METHODS
Surgical material from lung adenocarcinoma from 12 patients who had a new coronavirus infection and from 12 patients who did not have COVID19 was examined. Analysis of clinical and anamnestic data, macroscopic and microscopic examination of tumor samples and adjacent intact tissue, immunohistochemical reactions using antibodies to virus proteins and CSC markers ALDH1, CD133 and CD34 were performed.
RESULTS
Adenocarcinoma samples from patients in the main group showed a significant increase in the number of cells expressing the CSCs markers ALDH1, CD133 and CD34 compared to adenocarcinoma samples from control group patients without SARS-CoV2 infection. We found an increase in the number of CSCs in patients with adenocarcinoma metastasis in lymph nodes in both the main and control groups. CSCs of lung adenocarcinomas from SARS-CoV2 survivors contain virus proteins Nucleocapside and Spike protein.
CONCLUSIONS
We found an increase in the number of CSCs with expression of ALDH1, CD133 and CD34 in lung adenocarcinoma in patients with new coronavirus infection. Increased number of ALDH1+, CD133+ CD34+ CSCs in tumor tissue enhance the metastatic potential of lung adenocarcinoma. The Nucleocapsid and Spike proteins of SARS-CoV2 virus are detectable in lung tissue from patients with new coronavirus infection, both in adenocarcinoma cells, CSCs, and in type II pneumocytes, macrophages, and endothelial cells, suggesting prolonged persistence of the virus proteins and probably the virus.
未标注
由于肿瘤细胞关键群体——癌症干细胞（CSCs）的生物学特性，肺癌在全球癌症发病率和死亡率结构中占据主导地位。严重急性呼吸综合征冠状病毒2（SARS-CoV2）对肿瘤CSCs及其微环境的影响尚未得到研究。
目的
研究新型冠状病毒肺炎（COVID-19）后肺腺癌中的CSCs。
材料与方法
检查了12例感染新型冠状病毒患者和12例未感染COVID-19患者的肺腺癌手术材料。进行了临床和既往史数据分析、肿瘤样本及相邻完整组织的大体和显微镜检查、使用针对病毒蛋白和CSC标志物醛脱氢酶1（ALDH1）、CD133和CD34的抗体的免疫组化反应。
结果
与未感染SARS-CoV2的对照组患者的腺癌样本相比，主要组患者的腺癌样本中表达CSC标志物ALDH1、CD133和CD34的细胞数量显著增加。我们发现主要组和对照组中伴有淋巴结转移的腺癌患者的CSCs数量均增加。SARS-CoV2幸存者的肺腺癌CSCs含有病毒核衣壳蛋白和刺突蛋白。
结论
我们发现新型冠状病毒感染患者的肺腺癌中，表达ALDH1、CD133和CD34的CSCs数量增加。肿瘤组织中ALDH1 +、CD133 +、CD34 + CSCs数量的增加增强了肺腺癌的转移潜能。在新型冠状病毒感染患者的肺组织中，无论是腺癌细胞、CSCs，还是II型肺泡上皮细胞、巨噬细胞和内皮细胞中，均可检测到SARS-CoV2病毒的核衣壳蛋白和刺突蛋白，这表明病毒蛋白可能还有病毒持续存在时间延长。
Zotero 插件