Tsunekawa Eisuke, Fujita Makoto, Sawada Tomohisa
Department of Applied Chemistry, School of Engineering, The University of Tokyo Mitsui Link Lab Kashiwanoha 1, FS CREATION, 6-6-2 Kashiwanoha, Kashiwa, Chiba, 277-0882, Japan.
Tokyo College, Institutes for Advanced Study, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
Angew Chem Int Ed Engl. 2025 Jan 21;64(4):e202416442. doi: 10.1002/anie.202416442. Epub 2024 Nov 14.
Methods for precisely constructing a β-sheet assembly with number-defined strands in solution remains quite limited due to its intense aggregation property. Here, we report the precise construction of a four-stranded anti-parallel β-sheet by utilizing a non-covalent approach. This was achieved by folding and assembly of Ag and a pentapeptide (1) with the Ala-3pa-Gly-3pa-Val (3pa: β-(3-pyridyl)-alanine) sequence, which was designed to form an interlocking Ag(1) ring through metal cross-linking of the side chains. NMR analyses and X-ray crystallographic studies characterized the structure of the discrete β-sheet assembly as well as the remarkable structural selectivity in terms of strands' number, orientation and the sheet type.
由于其强烈的聚集特性，在溶液中精确构建具有数量确定链的β-折叠组装体的方法仍然非常有限。在此，我们报告了通过利用非共价方法精确构建四链反平行β-折叠。这是通过折叠和组装银与具有Ala-3pa-Gly-3pa-Val（3pa：β-(3-吡啶基)-丙氨酸）序列的五肽（1）实现的，该序列被设计通过侧链的金属交联形成互锁的Ag(1)环。核磁共振分析和X射线晶体学研究表征了离散β-折叠组装体的结构以及在链的数量、取向和折叠类型方面显著的结构选择性。
