Zeman E M, Bedford J S
Int J Radiat Oncol Biol Phys. 1986 Jan;12(1):51-8. doi: 10.1016/0360-3016(86)90415-3.
Co-cultured C3H 10T1/2 cells, in which transformed cells were grown as discrete colonies on top of density-inhibited monolayers of untransformed cells, were used to determine the potential usefulness of a short term assay system for the study of differential radiation effects as they may apply to cell populations with differing turnover rates, but in close physical contact. Mixed cultures were exposed to either an acute dose of 20 Gy of Cs-137 gamma rays, or a dose of 72 Gy delivered at a low dose rate of 0.34 Gy per hour. These treatments resulted in approximately equal levels of damage to the untransformed monolayers. At 10-day intervals after treatment, representative flasks from each dose group were examined for evidence of degeneration, and subsequent regrowth, if any, of the transformed colonies and untransformed monolayers. For comparable amounts of visible damage to the untransformed monolayers, the low dose rate irradiation was more effective at delaying regrowth of, or even eradicating, transformed colonies. These results are consistent with expectations based on previous results in which dose-rate or dose fractionation isoeffect curves were compared for these two cell types, grown independently in plateau phase cultures.
共培养的C3H 10T1/2细胞（其中转化细胞在未转化细胞的密度抑制单层细胞上形成离散集落生长）用于确定短期检测系统在研究不同辐射效应方面的潜在用途，这些效应可能适用于具有不同更新率但紧密物理接触的细胞群体。将混合培养物暴露于20 Gy的Cs-137γ射线急性剂量，或每小时0.34 Gy的低剂量率下给予72 Gy的剂量。这些处理对未转化的单层细胞造成的损伤程度大致相同。在处理后的10天间隔期，检查每个剂量组的代表性培养瓶，以寻找退化的证据，以及转化集落和未转化单层细胞随后是否有再生长（如果有的话）。对于未转化单层细胞相当程度的可见损伤，低剂量率照射在延迟转化集落的再生长甚至根除转化集落方面更有效。这些结果与基于先前结果的预期一致，在先前的结果中，比较了这两种细胞类型在平台期培养中独立生长时的剂量率或剂量分割等效效应曲线。
