Urano M, Kahn J
Int J Radiat Oncol Biol Phys. 1986 Jan;12(1):89-93. doi: 10.1016/0360-3016(86)90420-7.
The kinetics of thermotolerance were studied in mouse normal and tumor tissues. Early generation isotransplants of a spontaneous fibrosarcoma, FSa-II, were used. Tumor cell suspension was transplanted into the C3Hf/Sed mouse foot. Hyperthermia was given by immersing animal feet into a water bath. The TG (tumor growth) time, the time required for half the treated tumors to reach 1000 mm3 from the first treatment day, was the end point. Foot reaction was studied as a normal tissue response. The RD50, the treatment time that induces a loss of one toe or greater reaction in half the treated animals, was analyzed. Thermotolerance developed rapidly and extensively in normal and tumor tissues. A significant difference was observed in the decay of thermotolerance between these tissues. The decay of thermal resistance was incomplete in the murine foot tissue, even at 14 days after the initial heat treatment. Although some experimental difficulties were involved in the tumor study, present results suggest a complete decay of thermotolerance in the FSa-II tumor in 8 days after a 7.5 min treatment of 45.5 degrees C. Thermal resistance again developed in both tissues following the second heat treatment, which was given 7 or 8 days after the first heat treatment. The development, maximum magnitude and decay of the second thermal resistance was comparable to those of the first thermal resistance in each tissue.
对小鼠正常组织和肿瘤组织的热耐受动力学进行了研究。使用了自发纤维肉瘤FSa-II的早期传代同基因移植瘤。将肿瘤细胞悬液接种到C3Hf/Sed小鼠的足部。通过将动物足部浸入水浴中来进行热疗。以TG（肿瘤生长）时间作为终点，TG时间是指从首次治疗日起，半数治疗肿瘤达到1000立方毫米所需的时间。将足部反应作为正常组织反应进行研究。分析了RD50，即导致半数治疗动物出现一个脚趾缺失或更严重反应的治疗时间。正常组织和肿瘤组织中均迅速且广泛地产生了热耐受。观察到这些组织在热耐受衰减方面存在显著差异。即使在首次热处理后14天，小鼠足部组织中的热抗性衰减仍不完全。尽管肿瘤研究存在一些实验困难，但目前的结果表明，在45.5摄氏度下进行7.5分钟治疗后，FSa-II肿瘤在8天内热耐受完全衰减。在首次热处理后7或8天进行第二次热处理后，两种组织中又再次产生了热抗性。第二次热抗性的产生、最大程度及衰减在每个组织中均与第一次热抗性相当。
