Zhang Jiajia, Shang Shenglan, Liu Wanbing, Cheng Yangyang, Hu Fan, Cao Zhengwang, Yue Ling, Xiang GuangDa, Li Tao
Department of Endocrinology, General Hospital of Central Theater Command, Wuhan, Hubei Province, 430070, China.
Department of Clinical Pharmacy, General Hospital of Central Theater Command, Wuhan, Hubei Province, 430070, China.
Future Sci OA. 2024 Dec 31;10(1):2413827. doi: 10.1080/20565623.2024.2413827. Epub 2024 Oct 23.
Platelet-rich plasma (PRP), enriched with multiple growth factors, is a promising adjunctive therapy for diabetic foot ulcers (DFUs). As a classic anti-platelet drug for diabetic patients, the effects of aspirin on the content of growth factors in PRP remains unclear. Our study enrolled diabetic patients who were currently taking or not taking aspirin as the research subjects, with healthy volunteers as the control. PRP from these individuals was activated with glucose calcium and thrombin. Growth factors levels in PRP activated supernatant (PRP-AS) and wound healing ability of platelet gel (PG) in the full-thickness skin defect diabetic mouse model were compared. We found the level of growth factors in PRP-AS derived from two groups of diabetic patients were not statistically different, whereas both lower than that from healthy volunteers. Similarly, we found better wound healing ability of PG from healthy volunteers than those from diabetic patients, but no difference between the two groups of diabetic patients in the mouse model. Aspirin does not interfere with autologous PRP therapy when using calcium gluconate and thrombin as agonists. However considering the content of growth factors, PRP from healthy volunteers is a preferable option for promoting DFU repair.
富含多种生长因子的富血小板血浆（PRP）是一种治疗糖尿病足溃疡（DFU）的有前景的辅助疗法。作为糖尿病患者的经典抗血小板药物，阿司匹林对PRP中生长因子含量的影响尚不清楚。我们的研究将目前正在服用或未服用阿司匹林的糖尿病患者作为研究对象，以健康志愿者作为对照。用葡萄糖酸钙和凝血酶激活这些个体的PRP。比较了PRP激活上清液（PRP-AS）中的生长因子水平以及全层皮肤缺损糖尿病小鼠模型中血小板凝胶（PG）的伤口愈合能力。我们发现两组糖尿病患者来源的PRP-AS中的生长因子水平无统计学差异，但均低于健康志愿者来源的PRP-AS。同样，我们发现健康志愿者来源的PG在小鼠模型中的伤口愈合能力优于糖尿病患者来源的PG，但两组糖尿病患者来源的PG之间无差异。当使用葡萄糖酸钙和凝血酶作为激动剂时，阿司匹林不干扰自体PRP治疗。然而，考虑到生长因子的含量，健康志愿者来源的PRP是促进DFU修复的更优选择。
