硼和含锂药物在体外皮肤黑素瘤细胞中的细胞毒性和积累的比较研究。
Comparative Study of Cytotoxicity and Accumulation of Boron and Lithium-Containing Drugs in Skin Melanoma Cells In Vitro.
机构信息
Budker Institute of Nuclear Physics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
Research Institute of Clinical and Experimental Lymphology - Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
出版信息
Bull Exp Biol Med. 2024 Oct;177(6):736-740. doi: 10.1007/s10517-024-06260-3. Epub 2024 Oct 23.
We studied cytotoxicity and accumulation of boron and lithium by cultured human fibroblasts and human and mouse skin melanoma cell cultures. The cytotoxicity of boron and lithium drugs was assessed by MTT tests in the boron and lithium concentration range of 10-640 μg/ml. Cell viability was significantly reduced after incubation with boron and lithium at concentrations >160 μg/ml. To assess accumulation of boron and lithium, the concentration of elements was measured using inductively coupled plasma atomic emission spectrometry. Melanoma cells more intensively accumulated lithium in comparison with boron. The results indicate the possibility of safe application of lithium salts in concentrations minimally required for successful neutron capture therapy.
我们研究了培养的人成纤维细胞和人及鼠皮肤黑色素瘤细胞培养物中硼和锂的细胞毒性和积累情况。通过 MTT 试验，在硼和锂浓度范围为 10-640μg/ml 的情况下，评估了硼和锂药物的细胞毒性。在浓度大于 160μg/ml 的硼和锂孵育后，细胞活力显著降低。为了评估硼和锂的积累情况，使用电感耦合等离子体原子发射光谱法测量了元素的浓度。与硼相比，黑色素瘤细胞更强烈地积累了锂。结果表明，在成功进行中子俘获治疗所需的最小浓度下，安全应用锂盐是可能的。
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