School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450001, China.
National Engineering Research Center of Wheat and Corn Further Processing, Henan University of Technology, Zhengzhou 450001, China.
Langmuir. 2024 Nov 5;40(44):23279-23288. doi: 10.1021/acs.langmuir.4c02599. Epub 2024 Oct 23.
Surface-imprinted polymers (ZIF-67@MIPs) supported by ZIF-67 were prepared by precipitation polymerization using enrofloxacin (ENR) as the template molecule, methacrylic acid as the functional monomer, and ethylene glycol dimethacrylate as the cross-linker. ZIF-67@MIPs were characterized by Fourier transform infrared spectrometry, X-ray diffraction, scanning electron microscopy, and particle size distribution. The adsorption performance of the polymer was studied. The adsorption equilibrium was reached within 30 min. The maximum adsorption was 9.02 μg·mg. The imprinting factor was 2.58. The polymer was then used as a sorbent of a solid-phase extraction column for the separation and purification of ENR in real water samples. The extraction conditions were optimized. The method was established by high-performance liquid chromatography, and the linearity was verified by UPLC-MSMS. The correlation coefficient and limits of detection and quantification were 0.9999, 0.23 ng·mL, and 0.76 ng·mL, respectively. The recoveries were in the range of 83.79-100.68%; the relative standard deviation was 4.46-7.35%. The above data indicated that the method could be used for the separation and enrichment of ENR in real samples.
表面印迹聚合物（ZIF-67@MIPs）通过沉淀聚合制备，以恩诺沙星（ENR）为模板分子，甲基丙烯酸为功能单体，乙二醇二甲基丙烯酸酯为交联剂。通过傅里叶变换红外光谱、X 射线衍射、扫描电子显微镜和粒径分布对 ZIF-67@MIPs 进行了表征。研究了聚合物的吸附性能。吸附平衡在 30 分钟内达到。最大吸附量为 9.02 μg·mg。印迹因子为 2.58。然后，将聚合物用作固相萃取柱的吸附剂，用于分离和纯化实际水样中的恩诺沙星。优化了萃取条件。通过高效液相色谱法建立了方法，并通过 UPLC-MSMS 验证了线性关系。相关系数和检测限及定量限分别为 0.9999、0.23 ng·mL 和 0.76 ng·mL。回收率在 83.79-100.68%之间；相对标准偏差为 4.46-7.35%。上述数据表明，该方法可用于实际样品中恩诺沙星的分离和富集。
