Bartold P M, Boyd R R, Page R C
J Cell Physiol. 1986 Jan;126(1):37-46. doi: 10.1002/jcp.1041260106.
The proteoglycans synthesized by fibroblasts derived from human donors of ages ranging from 12 years to 68 years have been studied. In addition, the in vitro proliferation rates of the various cell strains were studied and demonstrated that increasing donor age correlated with a decrease in proliferative activity. The incorporation of [35S]-sulfate into proteoglycans decreased with increasing donor age with cells from the oldest donor demonstrating a 50% reduction compared with cells from the youngest donor. Analysis on Sepharose CL-4B of isolated [35S]-labeled proteoglycans for molecular size distribution revealed few differences between the cell-layer-associated proteoglycans of all cell strains studied. However, analysis of the medium-associated [35S]-labeled proteoglycans demonstrated an increase in the amount of small molecular size proteoglycans with increasing age. More specific analysis of the glycosaminoglycan composition revealed an increase in heparan sulfate from 52% to 73% in the cell-layer-associated proteoglycans of cells from the youngest and oldest donors, respectively. Accompanying this increase was a relative decrease in dermatan and chondroitin sulfate content from 24% to 13% and 25% to 16%, respectively, with increasing donor age. Additionally, the degree of N-sulfation of cell layer heparan sulfate increased with age. Heparan sulfate levels increased in the medium as well with increasing age, with a concomitant decrease in chondroitin sulfate. The quantity of medium-derived dermatan sulfate remained relatively evenly distributed throughout the various ages studied. The various differences noted are considered to reflect the general metabolic changes associated with aging. In particular the increase in heparan sulfate content with age is considered to be related to the decreased proliferative activity of the fibroblasts with increasing age.
对来自年龄在12岁至68岁之间人类供体的成纤维细胞合成的蛋白聚糖进行了研究。此外，还研究了各种细胞株的体外增殖率，结果表明供体年龄的增加与增殖活性的降低相关。随着供体年龄的增加，[35S] - 硫酸盐掺入蛋白聚糖的量减少，来自最年长供体的细胞与最年轻供体的细胞相比，减少了50%。对分离的[35S] - 标记蛋白聚糖进行Sepharose CL - 4B分析以确定分子大小分布，结果显示在所研究的所有细胞株的细胞层相关蛋白聚糖之间几乎没有差异。然而，对培养基相关的[35S] - 标记蛋白聚糖的分析表明，随着年龄的增加，小分子大小蛋白聚糖的量增加。对糖胺聚糖组成的更具体分析显示，在来自最年轻和最年长供体细胞的细胞层相关蛋白聚糖中，硫酸乙酰肝素分别从52%增加到73%。随着供体年龄的增加，伴随这种增加的是硫酸皮肤素和硫酸软骨素含量分别从24%相对降低到13%以及从25%相对降低到16%。此外，细胞层硫酸乙酰肝素的N - 硫酸化程度随着年龄的增加而增加。随着年龄的增加，培养基中硫酸乙酰肝素水平也增加，同时硫酸软骨素减少。培养基来源的硫酸皮肤素的量在整个研究的不同年龄中保持相对均匀分布。所观察到的各种差异被认为反映了与衰老相关的一般代谢变化。特别是随着年龄的增加硫酸乙酰肝素含量的增加被认为与成纤维细胞随着年龄增加而降低的增殖活性有关。
