Key Laboratory of Multi-Cell Systems, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai 200031, China.
Yi Chuan. 2024 Oct;46(10):779-794. doi: 10.16288/j.yczz.24-203.
With the rapid development of high-throughput sequencing technology in the past decade, an increasing number of sequencing methods targeting different types of DNA damage have been developed and widely used in the field. These technologies not only help to elucidate the dynamic processes of repair pathways corresponding to different types of lesions, understand the underlying mechanisms of key factors and identify new hotspots prone to damage, but also greatly advanced our knowledge of crucial physiological processes such as meiotic homologous recombination, antibody generation and cytosine demethylation. These advancements hold significant potential for broader applications in exploring disease initiation and drug development. However, understanding and selecting the appropriate techniques have become difficult. This article reviews the main sequencing detection methods for the most common DNA lesions and introduce their principles, thereby providing valuable insights for the selection, application, further development and optimization of these technologies.
在过去十年中，高通量测序技术迅速发展，针对不同类型 DNA 损伤的测序方法层出不穷，并广泛应用于该领域。这些技术不仅有助于阐明不同损伤类型修复途径的动态过程，理解关键因素的潜在机制和鉴定新的易损热点，而且极大地推进了我们对减数分裂同源重组、抗体生成和胞嘧啶去甲基化等关键生理过程的认识。这些进展为探索疾病发生和药物开发提供了更广泛的应用潜力。然而，理解和选择合适的技术变得困难起来。本文综述了最常见 DNA 损伤的主要测序检测方法，并介绍了它们的原理，为这些技术的选择、应用、进一步发展和优化提供了有价值的参考。
