Department of Agricultural Animals and Nematodes, Faculty of Agriculture (Girls Branch), Al-Azhar University, Cairo, Egypt.
Department of Biology, University College in Darb, Jazan University, Al-Darb, 45142, Jazan, Saudi Arabia.
Sci Rep. 2024 Oct 23;14(1):24999. doi: 10.1038/s41598-024-70391-w.
This study aimed to examine whether or not aluminum chloride (AlCl)-induced hepatotoxicity might be mitigated using magnetic water (MW) in rats. This study involved 28 adult male rats randomly assigned into the following 4 groups (7 rats/group): normal control (Cnt), MW, AlCl, and Al Cl + MW. The Cnt group orally received normal saline, the MW group drank MW ad libitum for 2 months, and the AlCl and AlCl + MW groups were orally administered AlCl (40 mg/kg b.w.) alone or in combination with MW for 2 months, respectively. MW reduced AlCl toxicity as proved at functional, molecular, and structural levels. Functionally, MW reduced serum levels of liver enzymes (ALT, AST, ALP, GGT), while increased total proteins, and albumin. MW also restored redox balance in the liver (lower MDA levels, higher activities of CAT and SOD enzymes, and upregulated expression of NrF2, HO-1, and GST genes. Molecularly, MW downregulated hepatic expression of the epigenetic (HDAC3), inflammatory (IL1β, TNFα, NFκβ), and endoplasmic reticulum stress (XBP1, BIP, CHOP) genes. Structurally, MW enhanced liver histology. With these results, we could conclude that MW has the potential to ameliorate the hepatotoxic effects of AlCl through targeting oxidative stress, inflammation, epigenesis, and endoplasmic reticulum stress.
本研究旨在探讨氯化铝（AlCl）诱导的肝毒性是否可以通过大鼠的磁化水（MW）得到缓解。本研究涉及 28 只成年雄性大鼠，随机分为以下 4 组（每组 7 只）：正常对照组（Cnt）、MW 组、AlCl 组和 AlCl+MW 组。Cnt 组口服生理盐水，MW 组自由饮用 MW 水 2 个月，AlCl 组和 AlCl+MW 组分别单独或联合 MW 口服 AlCl（40mg/kg b.w.）2 个月。MW 在功能、分子和结构水平上减轻了 AlCl 的毒性。功能上，MW 降低了血清中肝脏酶（ALT、AST、ALP、GGT）的水平，同时增加了总蛋白和白蛋白的水平。MW 还恢复了肝脏的氧化还原平衡（降低 MDA 水平，提高 CAT 和 SOD 酶的活性，并上调 NrF2、HO-1 和 GST 基因的表达）。分子水平上，MW 下调了肝脏中表观遗传（HDAC3）、炎症（IL1β、TNFα、NFκβ）和内质网应激（XBP1、BIP、CHOP）基因的表达。结构上，MW 增强了肝脏组织学。综上所述，MW 通过靶向氧化应激、炎症、表观遗传和内质网应激，有可能减轻 AlCl 的肝毒性作用。
