Lauritzen E
J Biochem Biophys Methods. 1986 Jan;12(1-2):113-20. doi: 10.1016/0165-022x(86)90056-4.
A wire electrode arrangement for a horizontal electroblotting apparatus is described. The electrodes were mounted in electrode conduits built on perforated plexiglass plates. Electroblotting was carried out in a buffer tank. Human serum proteins were separated by sodium dodecyl sulfate--polyacrylamide gel electrophoresis and electrophoretically transferred onto nitrocellulose membranes. Gas bubbles developed by electrolysis at the lower electrode were trapped in electrode conduits and removed by circulating buffer. Accumulation of gas bubbles between the electrodes during operation was avoided.
描述了一种用于水平电印迹装置的线状电极装置。电极安装在基于多孔有机玻璃板构建的电极导管中。电印迹在缓冲罐中进行。人血清蛋白通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离，并通过电泳转移到硝酸纤维素膜上。在下部电极处通过电解产生的气泡被困在电极导管中，并通过循环缓冲液去除。避免了操作过程中电极之间气泡的积聚。
