Raheem Zainab J, Abdulbaqi Hayder Raad
Department of Periodontics, College of Dentistry, University of Baghdad, Baghdad, Iraq.
Int J Dent. 2024 Oct 15;2024:8006278. doi: 10.1155/2024/8006278. eCollection 2024.
This case-control study aimed to investigate the diagnostic accuracy of salivary interleukin (IL)-1, IL-10, and IL-1/IL-10 ratio to discriminate periodontitis from periodontal health and stable from unstable periodontitis. Saliva samples were collected from 135 (healthy on an intact periodontium = 45 [as healthy control], stable periodontitis = 39, and unstable periodontitis = 51) participants, and then clinical periodontal parameters were recorded. An enzyme-linked immunosorbent assay was used to determine salivary levels of IL-1 and IL-10. Area under the curves (AUCs), sensitivity, and specificity of IL-1, IL-10, and IL-1/IL-10 were estimated to discriminate between groups. The level of salivary IL-1 was significantly higher in unstable periodontitis than in stable periodontitis and healthy control groups (426 ± 59, 247 ± 55, and 204 ± 36 pg/ml [picograms per milliliter], respectively). While the level of salivary IL-10 was significantly higher in the control group (360.7 ± 80.5 pg/ml) than unstable periodontitis group (146.92 ± 1.8 pg/ml), no significant difference was found between the control and stable periodontitis (317.04 ± 59.8 pg/ml) groups. IL-1, IL-10, and IL-1/IL-10 had significant diagnostic accuracy for differentiating healthy control from unstable periodontitis (AUCs = 0.99, 0.96, and 1; sensitivity = 0.98,1, and 1; specificity = 0.95, 0.95, and 1, respectively). Similarly, they showed significant diagnostic accuracy in distinguishing unstable from stable periodontitis (AUCs = 0.98, 0.99, and 1; sensitivity = 0.94, 1, and 1; specificity = 0.94, 0.97, and 1, respectively). Salivary IL-1, IL-10, and IL-1/IL-10 have a high potential to discriminate healthy control from periodontitis and stable from unstable periodontitis. ClinicalTrials.gov identifier: NCT05722613.
这项病例对照研究旨在调查唾液白细胞介素（IL）-1、IL-10以及IL-1/IL-10比值在区分牙周炎与牙周健康状况、区分牙周炎稳定期与不稳定期方面的诊断准确性。从135名参与者中收集唾液样本（完整牙周组织健康者45名[作为健康对照]，牙周炎稳定期患者39名，牙周炎不稳定期患者51名），然后记录临床牙周参数。采用酶联免疫吸附测定法测定唾液中IL-1和IL-10的水平。估计IL-1、IL-10以及IL-1/IL-10的曲线下面积（AUC）、敏感性和特异性以区分不同组。唾液IL-1水平在牙周炎不稳定期显著高于牙周炎稳定期和健康对照组（分别为426±59、247±55和204±36皮克/毫升[每毫升皮克数]）。虽然唾液IL-10水平在对照组（360.7±80.5皮克/毫升）显著高于牙周炎不稳定期组（146.92±1.8皮克/毫升），但在对照组与牙周炎稳定期组（317.04±59.8皮克/毫升）之间未发现显著差异。IL-1、IL-10以及IL-1/IL-10在区分健康对照与牙周炎不稳定期方面具有显著的诊断准确性（AUC分别为0.99、0.96和1；敏感性分别为0.98、1和1；特异性分别为0.95、0.95和1）。同样，它们在区分牙周炎不稳定期与稳定期方面也显示出显著的诊断准确性（AUC分别为0.98、0.99和1；敏感性分别为0.94、1和1；特异性分别为0.94、0.97和1）。唾液IL-1、IL-10以及IL-1/IL-10在区分健康对照与牙周炎、区分牙周炎稳定期与不稳定期方面具有很高潜力。临床试验.gov标识符：NCT05722613。
