Christou Atalanti, Nikola Fotini, Goulas Vlasios
Department of Agricultural Sciences, Biotechnology & Food Science, Cyprus University of Technology, Lemesos, 3036, Cyprus.
Chem Biodivers. 2025 Mar;22(3):e202401337. doi: 10.1002/cbdv.202401337. Epub 2024 Nov 18.
The current work aims to optimize the ultrasound-assisted extraction (UAE) of Cistus salviifolius L. (aerial parts) antioxidative phenolic compounds using response surface methodology. A Box-Behnken design has been conducted to investigate the effect of four factors, namely: (i) percentage of ethanol (50-90 %, v/v), (ii) temperature (40-80 °C), (iii) solvent-solid ratio (10-50 mL g) and (iv) extraction time (5-25 min) on four responses, namely: total phenolic content (TPC), total flavonoid content (TFC) 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical inhibition, and ferric reducing antioxidant power (FRAP). Based on the desirability index, UAE with 50 % (v/v) ethanol, at 80 °C, using a solvent-solid ratio of 32.24 mL g, for 21 min resulted in the maximum recovery of phenolic antioxidants. Under optimum conditions, the experimental values of TPC, TFC, % DPPH radical scavenging activity, and FRAP were 171.67±4.69 mg GAE g, 26.87±0.78 mg CE g, 81.31±0.16 %, and 1038.22±7.69 μmol TE g, respectively. Results shows a reasonable agreement of experimental values with the predicted ones; the absolute error values being in all cases lower than 2.90 %. The present work provide a developed eco-friendly extarction method that is appropriate for the improved recovery of phenolic antioxidants from C. salviifolius L.
当前的工作旨在利用响应面法优化超声辅助提取（UAE）鼠尾草叶岩蔷薇（地上部分）抗氧化酚类化合物的工艺。采用Box-Behnken设计研究了四个因素的影响，即：（i）乙醇百分比（50-90%，v/v）、（ii）温度（40-80°C）、（iii）溶剂与固体比例（10-50 mL g）和（iv）提取时间（5-25分钟）对四个响应指标的影响，即：总酚含量（TPC）、总黄酮含量（TFC）、2,2-二苯基-1-苦基肼（DPPH）自由基抑制率和铁还原抗氧化能力（FRAP）。基于可取性指数，在80°C下使用32.24 mL g的溶剂与固体比例、50%（v/v）乙醇提取21分钟的超声辅助提取法可实现酚类抗氧化剂的最大回收率。在最佳条件下，TPC、TFC、DPPH自由基清除活性百分比和FRAP的实验值分别为171.67±4.69 mg GAE g、26.87±0.78 mg CE g、81.31±0.16%和1038.22±7.69 μmol TE g。结果表明实验值与预测值具有合理的一致性；所有情况下的绝对误差值均低于2.90%。本研究提供了一种改进的环保提取方法，适用于从鼠尾草叶岩蔷薇中提高酚类抗氧化剂的回收率。
