Gollins S W, Porterfield J S
J Gen Virol. 1986 Jan;67 ( Pt 1):157-66. doi: 10.1099/0022-1317-67-1-157.
Fusion between purified [3H]uridine-labelled West Nile virus (WNV) particles and liposomes containing RNase, was assayed by degradation of the viral RNA to trichloroacetic acid-soluble material. Fusion of virus with liposomes containing phosphatidylcholine, phosphatidylethanolamine, sphingomyelin and cholesterol (at a molar ratio of 1:1:1:1.5) was found to be dependent on pH with maximum fusion occurring at pH 6.7 and below. At pH 6.6 fusion was rapid and was essentially complete within 2 min at 37 degrees C. At this time, approximately 50% of the viral RNA had been degraded and increasing the concentration of liposomes or time allowed for fusion increased this percentage only slightly. Fusion was dependent on temperature, was almost totally non-leaky and was not dependent on the presence of divalent cations. The lipid composition of liposomes was found to influence both the pH optimum for fusion and the maximum degree of fusion observed. Electron microscopy was used to visualize the fusion reaction between liposomes and virus particles.
通过将病毒RNA降解为三氯乙酸可溶性物质，检测纯化的[3H]尿苷标记的西尼罗河病毒（WNV）颗粒与含有核糖核酸酶的脂质体之间的融合。发现病毒与含有磷脂酰胆碱、磷脂酰乙醇胺、鞘磷脂和胆固醇（摩尔比为1:1:1:1.5）的脂质体的融合取决于pH值，在pH 6.7及以下时融合达到最大值。在pH 6.6时，融合迅速，在37℃下2分钟内基本完成。此时，约50%的病毒RNA已被降解，增加脂质体浓度或融合时间只会使该百分比略有增加。融合取决于温度，几乎完全不渗漏，且不依赖于二价阳离子的存在。发现脂质体的脂质组成会影响融合的最佳pH值和观察到的最大融合程度。使用电子显微镜观察脂质体与病毒颗粒之间的融合反应。
