Ishiguro Kei-Ichiro
Department of Chromosome Biology, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan.
J Biochem. 2025 Jan 6;177(1):1-3. doi: 10.1093/jb/mvae072.
The nuclear envelope (NE) is a double-membrane structure critical for genome maintenance and cellular function, composed of the inner and outer nuclear membranes. In fission yeast, the inner nuclear membrane (INM) proteins Lem2 and Bqt4 are essential for maintaining NE integrity. The study published by Hiraoka group explores the interactions between Lem2 and Bqt4 with lipid synthesis enzymes, addressing their roles in NE maintenance. The authors identified Lem2- and Bqt4-binding proteins using immunoprecipitation and mass spectrometry, revealing that Lem2 interacts with lipid synthesis enzymes, whilst Bqt4 binds to an enzyme that involves in glucosylceramide synthesis. These findings suggest that Lem2 and Bqt4 independently contribute to NE structure and its integrity through distinct lipid metabolic pathways, highlighting their complementary roles in nuclear membrane homeostasis. This study represents a significant step forward in the field of NE biology to unravel the complexities of nuclear membrane dynamics.
核膜（NE）是一种对基因组维持和细胞功能至关重要的双膜结构，由内核膜和外核膜组成。在裂殖酵母中，内核膜（INM）蛋白Lem2和Bqt4对于维持核膜完整性至关重要。平冈小组发表的这项研究探讨了Lem2和Bqt4与脂质合成酶之间的相互作用，阐述了它们在核膜维持中的作用。作者通过免疫沉淀和质谱法鉴定了与Lem2和Bqt4结合的蛋白，发现Lem2与脂质合成酶相互作用，而Bqt4则与一种参与葡糖神经酰胺合成的酶结合。这些发现表明，Lem2和Bqt4通过不同的脂质代谢途径独立地对核膜结构及其完整性做出贡献，突出了它们在核膜稳态中的互补作用。这项研究代表了核膜生物学领域在揭示核膜动力学复杂性方面向前迈出的重要一步。
