Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing 100029, China.
State Key Laboratory of Heavy Oil Processing, State Key Laboratory of Petroleum Pollution Control, China University of Petroleum-Beijing, Beijing 102249, China.
J Agric Food Chem. 2024 Nov 6;72(44):24857-24865. doi: 10.1021/acs.jafc.4c04976. Epub 2024 Oct 24.
Emerging perfluoroalkyl and polyfluoroalkyl substances (PFASs) have received much attention in recent years. Milk has usually low concentrations of PFASs, but it remains an important PFAS exposure route due to its high consumption. In this study, a method for simultaneous determination of 60 PFASs in milk was developed by online TurboFlow liquid chromatography-tandem mass spectrometry. A pH-dependent cold-induced liquid-liquid extraction was developed for precleanup and enrichment. Method quantification limits ranged from 0.003 to 0.500 ng/mL. For PFASs without corresponding isotope-labeled internal standards, Tanimoto coefficient and logP were introduced for the selection of internal standards. The matrix spiked recoveries ranged from 47 to 190%. The method was applied for PFAS analysis in 35 milk samples. PFBS, perfluorohexanesulfonic acid, perfluorooctanesulfonic acid, PFNS, PFPeA, perfluorooctanoic acid, PFNA, and 6:2 FTS were detected. The developed online TurboFlow method was simple, reliable, efficient, and applicable for trace analysis of PFASs in milk.
近年来，新兴的全氟烷基和多氟烷基物质（PFASs）受到了广泛关注。牛奶中的 PFASs 浓度通常较低，但由于其高消耗量，仍然是 PFASs 的重要暴露途径。在这项研究中，通过在线 TurboFlow 液相色谱-串联质谱法开发了一种同时测定牛奶中 60 种 PFASs 的方法。建立了一种 pH 依赖的冷诱导液-液萃取方法用于预净化和富集。方法的定量限范围为 0.003 至 0.500ng/mL。对于没有相应同位素标记内标物的 PFASs，引入了 Tanimoto 系数和 logP 来选择内标物。基质加标回收率范围为 47%至 190%。该方法应用于 35 个牛奶样品中的 PFAS 分析。检测到 PFBS、全氟己烷磺酸、全氟辛烷磺酸、PFNS、PFPeA、全氟辛酸、PFNA 和 6:2 FTS。开发的在线 TurboFlow 方法简单、可靠、高效，适用于牛奶中痕量 PFASs 的分析。
