Department of Plant Breeding and Biotechnology (PBB), Faculty of Agriculture, University of Zabol, Zabol, Iran.
Department of Plant Breeding and Biotechnology (PBB), Faculty of Agriculture, University of Zabol, Zabol, Iran.
Gene. 2025 Jan 20;934:149029. doi: 10.1016/j.gene.2024.149029. Epub 2024 Oct 22.
Grapes are considered a crucial fruit crop with extensive uses globally. Cluster compactness is an undesirable trait for the productivity of Yaghooti grape, and it reduces its desirability among consumers. The RNA-Seq data were analyzed in three stages of cluster development using the FEELnc software, leading to the identification of 849 lncRNAs. 183 lncRNAs were differentially expressed during cluster development stages. The GO and KEGG enrichment analyses of these lncRNAs revealed that they target 1,814 genes, including CKX, PG, PME, NPC2, and UGT. The analysis demonstrated a relationship between these lncRNAs and key processes such as grape growth and development, secondary metabolite synthesis, and resistance to both biotic and abiotic stresses. These findings, combined with molecular experiments on lncRNA interactions with other regulatory factors, could enhance Yaghooti grape quality and decrease cluster compactness.
葡萄被认为是一种重要的水果作物，在全球范围内有广泛的用途。对于 Yaghooti 葡萄的生产力来说，果穗紧凑是一种不理想的特性，这降低了其在消费者中的受欢迎程度。使用 FEELnc 软件对果穗发育的三个阶段的 RNA-Seq 数据进行了分析，共鉴定出 849 个 lncRNA。在果穗发育阶段，有 183 个 lncRNA 表现出差异表达。对这些 lncRNA 的 GO 和 KEGG 富集分析表明，它们靶向 1814 个基因，包括 CKX、PG、PME、NPC2 和 UGT。分析表明，这些 lncRNA 与葡萄生长发育、次生代谢物合成以及对生物和非生物胁迫的抗性等关键过程之间存在关系。这些发现，结合对 lncRNA 与其他调控因子相互作用的分子实验，可能会提高 Yaghooti 葡萄的品质并降低果穗紧凑度。
