Post-Graduate Program in Adult Health (PPGSAD), Federal University of Maranhão (UFMA), São Luis, Brazil.
Service of Endocrinology, University Hospital of the Federal University of Maranhao (HUUFMA), São Luis, Brazil.
Front Endocrinol (Lausanne). 2024 Oct 10;15:1481050. doi: 10.3389/fendo.2024.1481050. eCollection 2024.
Pituitary adenomas (PAs) are benign tumors with high prevalence and, occasionally, aggressive course. The tumorigenesis of these lesions is not completely understood at the molecular level. BAK1 and BAX proteins play fundamental roles in apoptosis and seem to interact with VDAC proteins, whose expressions have been markedly altered in cancer, impacting their prognosis.
to evaluate the gene expression of and and their association with clinical and imaging characteristics in PA.
Clinical-epidemiological data were collected from 117 tumor samples from patients affected by PA. Invasiveness was assessed by the Knosp scale. Gene expression was examined by real-time PCR. Relative expression analysis was performed by 2^(-DDCt) method.
The sample was mainly composed of women (69/117 - 57.2%). Tumor subtypes observed were Non-Functioning (NF) (73/117 - 62.4%), Acromegaly (24/117 - 20.5%) and Cushing's Disease (CD) (20/117 - 17.1%). Compared to normal tissue, there was a significant reduction in expression in the Acromegaly (p=0.029) and NF (p=0.002) groups. expression was lower in all groups (p <0.001; p=0.007; P =0.005). No difference was found in and expression, compared to normal pituitary. Overexpression of occurred in PAs with post-surgical regrowth (p=0.042). A strongly negative correlation was observed in and expression in CD.
The results indicated that downregulations of and may be related to resistance to apoptosis. In contrast, overexpression of in regrowing PAs suggests an antiapoptotic role for this gene. In summary, the genes evaluated might be involved in the biopathology of PAs.
垂体腺瘤（PA）是一种常见的良性肿瘤，偶尔会出现侵袭性病程。这些病变的肿瘤发生机制在分子水平上尚未完全阐明。BAK1 和 BAX 蛋白在细胞凋亡中发挥着重要作用，并且似乎与 VDAC 蛋白相互作用，后者在癌症中的表达发生了明显改变，影响了其预后。
评估 和 在 PA 中的基因表达及其与临床和影像学特征的相关性。
从 117 例 PA 患者的肿瘤样本中收集临床流行病学数据。通过 Knosp 量表评估侵袭性。通过实时 PCR 检查基因表达。通过 2^(-DDCt)方法进行相对表达分析。
样本主要由女性组成（69/117-57.2%）。观察到的肿瘤亚型是非功能性（NF）（73/117-62.4%）、肢端肥大症（24/117-20.5%）和库欣病（CD）（20/117-17.1%）。与正常组织相比，肢端肥大症（p=0.029）和 NF 组（p=0.002）中 的表达显著降低。所有组的 表达均降低（p<0.001;p=0.007;p=0.005）。与正常垂体相比， 与 表达无差异。术后复发的 PA 中 过表达（p=0.042）。CD 中观察到 与 表达呈强烈负相关。
结果表明， 和 的下调可能与抗细胞凋亡有关。相反，在复发性 PA 中 过表达表明该基因具有抗凋亡作用。综上所述，所评估的基因可能参与 PA 的生物病理学。
