Kleszcz Robert, Dorna Dawid, Stawny Maciej, Paluszczak Jarosław
Department of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, 60-806 Poznań, Poland.
Department of Pharmaceutical Biochemistry, Doctoral School, Poznan University of Medical Sciences, 60-806 Poznań, Poland.
Curr Issues Mol Biol. 2024 Sep 25;46(10):10731-10744. doi: 10.3390/cimb46100637.
The efficacy of treatment of head and neck squamous cell carcinoma (HNSCC) patients is still unsatisfactory, and there is an ongoing search for novel therapies. Locoregionally advanced HNSCC cases, which frequently require combined surgery and chemoradiotherapy, are especially difficult to treat. Natural compounds, like -derived lignans-honokiol (HON) and magnolol (MAG)-can reduce cancer cell growth but retain a good safety profile and thus may show benefit as adjuvant therapeutics. The aim of this study was to evaluate the anti-cancer effects of HON and MAG in HNSCC cell lines and compare their effects between cisplatin-sensitive and cisplatin-tolerant cells. Cell viability was evaluated in FaDu and SCC-040 cells growing as monolayers and as spheroids. The effect of HON and MAG on the cell cycle, apoptosis, and gene expression was compared between wild-type FaDu cells and cisplatin persister FaDu cells. We observed that HON and MAG were more potent in reducing cell viability in cisplatin persister FaDu cells, although this effect was not directly followed by increased rates of apoptosis. Thus, HON's and MAG's capacity to affect cisplatin persister cells needs further studies. In general, we observed that HON exerted stronger cytotoxic effects than MAG in HNSCC cells, and the difference in their anti-cancer activity was especially pronounced in cells cultured in 3D.
头颈部鳞状细胞癌（HNSCC）患者的治疗效果仍不尽人意，人们一直在寻找新的治疗方法。局部晚期HNSCC病例通常需要联合手术和放化疗，治疗起来尤其困难。天然化合物，如源自木脂素的和厚朴酚（HON）与厚朴酚（MAG），可以抑制癌细胞生长，但安全性良好，因此可能作为辅助治疗药物显示出益处。本研究的目的是评估HON和MAG对HNSCC细胞系的抗癌作用，并比较它们在顺铂敏感和顺铂耐受细胞之间的效果。在单层生长和形成球体的FaDu和SCC-040细胞中评估细胞活力。比较了HON和MAG对野生型FaDu细胞和顺铂持续存在的FaDu细胞的细胞周期、凋亡和基因表达的影响。我们观察到，HON和MAG在降低顺铂持续存在的FaDu细胞的细胞活力方面更有效，尽管这种效果并没有直接导致凋亡率增加。因此，HON和MAG影响顺铂持续存在细胞的能力需要进一步研究。总体而言，我们观察到HON在HNSCC细胞中比MAG具有更强的细胞毒性作用，并且它们的抗癌活性差异在三维培养的细胞中尤为明显。
