Zhou Fangwei, Xu Liang, Shi Congguang, Wu Fengying, Yang Shaozong
Zhejiang Key Laboratory of Forest Genetics and Breeding, Zhejiang Academy of Forestry, Hangzhou 310023, China.
Curr Issues Mol Biol. 2024 Sep 26;46(10):10779-10794. doi: 10.3390/cimb46100640.
Paper Mulberry () possesses medicinal, economic, and ecological significance and is extensively used for feed production, papermaking, and ecological restoration due to its ease of propagation, rapid growth rate, and strong stress resistance. The recent completion of the sequencing of the Paper Mulberry genome has prompted further research into the genetic breeding and molecular biology of this important species. A highly stable reference gene is essential to enhance the quantitative analysis of functional genes in Paper Mulberry; however, none has been identified. Accordingly, in this study, the leaves, stems, roots, petioles, young fruits, and mature fruits of Paper Mulberry plants were selected as experimental materials, and nine candidate reference genes, namely, , , , , , , , , and , were identified by RT-qPCR. Their stability was assessed using the geNorm, Normfinder, Delta Ct, BestKeeper, and RefFinder algorithms, identifying and as showing the greatest stability. The expression of , which regulates the production of trichomes, was examined in the leaves of plants of the wild type (which have more trichomes) and mutant (which have fewer trichomes) at various developmental stages to validate the results of this study. As a result, their identification addresses a critical gap in the field of Paper Mulberry research, providing a solid foundation for future research that will concentrate on the characterization of pertinent functional genes in this economically valuable species.
构树具有药用、经济和生态意义，因其易于繁殖、生长速度快和抗逆性强而被广泛用于饲料生产、造纸和生态修复。最近构树基因组测序的完成促使人们对这个重要物种的遗传育种和分子生物学进行进一步研究。一个高度稳定的内参基因对于增强构树中功能基因的定量分析至关重要；然而，尚未有内参基因被鉴定出来。因此，在本研究中，选取构树植株的叶片、茎、根、叶柄、幼果和成熟果实作为实验材料，通过RT-qPCR鉴定了9个候选内参基因，即……（此处原文未完整列出9个基因名称）。使用geNorm、Normfinder、Delta Ct、BestKeeper和RefFinder算法评估它们的稳定性，确定……（此处原文未明确写出具体基因）表现出最大的稳定性。在野生型（具有更多毛状体）和突变体（具有更少毛状体）植株不同发育阶段的叶片中检测了调控毛状体产生的……（此处原文未明确写出具体基因）的表达，以验证本研究的结果。结果，它们的鉴定填补了构树研究领域的一个关键空白，为未来专注于这个经济价值物种中相关功能基因表征的研究提供了坚实的基础。
