Dash Prasanta K, Rai Rhitu, Pradhan Sharat Kumar, Shivaraj Sheelavanta Matha, Deshmukh Rupesh, Sreevathsa Rohini, Singh Nagendra K
ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi 110012, India.
ICAR-National Rice Research Institute, Cuttack 753006, India.
Antioxidants (Basel). 2023 Apr 18;12(4):950. doi: 10.3390/antiox12040950.
Flax ( L.) is a self-pollinating, annual, diploid crop grown for multi-utility purposes for its quality oil, shining bast fiber, and industrial solvent. Being a cool (Rabi) season crop, it is affected by unprecedented climatic changes such as high temperature, drought, and associated oxidative stress that, globally, impede its growth, production, and productivity. To precisely assess the imperative changes that are inflicted by drought and associated oxidative stress, gene expression profiling of predominant drought-responsive genes (AREB, DREB/CBF, and ARR) was carried out by qRT-PCR. Nevertheless, for normalization/quantification of data obtained from qRT-PCR results, a stable reference gene is mandatory. Here, we evaluated a panel of four reference genes (, , , and ) and assessed their suitability as stable reference genes for the normalization of gene expression data obtained during drought-induced oxidative stress in flax. Taking together, from the canonical expression of the proposed reference genes in three different genotypes, we report that as a stand-alone and and in tandem are suitable reference genes to be used for the real-time visualization of cellular impact of drought and oxidative stress on flax.
亚麻（L.）是一种自花授粉的一年生二倍体作物，因其优质油、光亮的韧皮纤维和工业溶剂而具有多种用途。作为一种冷季（拉比）作物，它受到高温、干旱等前所未有的气候变化以及相关氧化应激的影响，在全球范围内阻碍其生长、产量和生产力。为了精确评估干旱和相关氧化应激所带来的必要变化，通过qRT-PCR对主要干旱响应基因（AREB、DREB/CBF和ARR）进行了基因表达谱分析。然而，为了对从qRT-PCR结果获得的数据进行标准化/定量，一个稳定的参考基因是必不可少的。在这里，我们评估了一组四个参考基因（、、和），并评估了它们作为稳定参考基因的适用性，用于标准化在亚麻干旱诱导的氧化应激期间获得的基因表达数据。综合来看，从所提出的参考基因在三种不同基因型中的典型表达，我们报告说，作为单独的参考基因，以及和串联使用时，是适用于实时可视化干旱和氧化应激对亚麻细胞影响的参考基因。
