Xue Qian, Yang Bingbing, Luo Kun, Luan Sheng, Kong Jie, Fu Qiang, Cao Jiawang, Chen Baolong, Dai Ping, Xing Qun, Li Xupeng, Meng Xianhong
State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao 266237, China.
Biology (Basel). 2024 Sep 24;13(10):758. doi: 10.3390/biology13100758.
C-type lectins are known for agglutination activity and play crucial roles in regulating the prophenoloxidase (proPO) activation system, enhancing phagocytosis and encapsulation, synthesizing antimicrobial peptides, and mediating antiviral immune responses. This work cloned a C-type lectin, ladderlectin (), from . comprised a 531 bp open reading frame (ORF) that encoded 176 amino acids. The predicted LvLL protein included a signal peptide and a CLECT domain. LvLL was predicted to feature a transmembrane region, suggesting it may be a transmembrane protein. was predominantly expressed in the shrimp's hepatopancreas. After WSSV infection, expression in the hepatopancreas increased significantly by 11.35-fold after 228 h, indicating a general upregulation. Knockdown of resulted in a significant decrease in WSSV viral load and a notable increase in shrimp survival rates. Additionally, knockdown of led to a significant downregulation of apoptosis-related genes and caspase 8 and a significant upregulation of and in WSSV-infected shrimp. This study showed that played a vital role in the interaction between . and WSSV.
C型凝集素以其凝集活性而闻名，在调节酚氧化酶原（proPO）激活系统、增强吞噬作用和包囊作用、合成抗菌肽以及介导抗病毒免疫反应中发挥关键作用。本研究从[具体来源]克隆了一种C型凝集素，即梯状凝集素（ladderlectin，LvLL）。LvLL包含一个531 bp的开放阅读框（ORF），编码176个氨基酸。预测的LvLL蛋白包括一个信号肽和一个CLECT结构域。LvLL被预测具有一个跨膜区域，表明它可能是一种跨膜蛋白。LvLL主要在虾的肝胰腺中表达。在感染白斑综合征病毒（WSSV）后，228小时后肝胰腺中LvLL的表达显著增加了11.35倍，表明普遍上调。敲低LvLL导致WSSV病毒载量显著降低，虾的存活率显著提高。此外，敲低LvLL导致WSSV感染的虾中凋亡相关基因[具体基因1]和半胱天冬酶8显著下调，以及[具体基因2]和[具体基因3]显著上调。本研究表明，LvLL在[具体生物]与WSSV之间的相互作用中起着至关重要的作用。
