Herrera-Castillo Lisbeth, Vallejo-Palma Germán, Saiz Nuria, Sánchez-Jiménez Abel, Isorna Esther, Ruiz-Jarabo Ignacio, de Pedro Nuria
Department of Genetics, Physiology and Microbiology, Faculty of Biological Sciences, University Complutense of Madrid, 28040 Madrid, Spain.
Department of Biodiversity, Ecology and Evolution, Faculty of Biological Sciences, University Complutense of Madrid, 28040 Madrid, Spain.
Biology (Basel). 2024 Oct 9;13(10):804. doi: 10.3390/biology13100804.
This study examined the metabolic rate (MO, oxygen consumption) of goldfish () under normal management conditions in aquaculture. Using an intermittent respirometry system, we assessed daily variations and the effects of feeding, handling, temperature increase, and anesthetics. MO exhibited a daily rhythm, with higher values during day. Feeding to satiety produced a 35% increase in MO compared to fasted animals, with a maximum peak after 3 h and returning to baseline after 7 h. Handling stress (5 min) produced a 140% MO peak (from 180 to 252 mg O kg h), returning to the routine MO after 2.5 h. An increase in water temperature (+0.1 °C min) up to 30 °C caused MO to peak at 200% after 2.5 h from the start of the temperature increase. The use of common anesthetics in aquaculture (MS-222, 2-phenoxyethanol and clove oil in deep anesthesia concentration) affects MO during the first few minutes after anesthetic recovery, but also during the following 4 h. It can be concluded that the metabolic rate is a good indicator of the goldfish's response to aquaculture practices involving energy expenditure and stress. Thus, intermittent respirometry is a valuable non-invasive tool for understanding and improving fish welfare in aquaculture.
本研究考察了水产养殖正常管理条件下金鱼的代谢率（MO，耗氧量）。我们使用间歇式呼吸测量系统评估了其每日变化以及投喂、处理、温度升高和麻醉剂的影响。代谢率呈现出每日节律，白天的值更高。与禁食动物相比，饱食投喂使代谢率提高了35%，3小时后达到最大峰值，7小时后恢复到基线水平。处理应激（5分钟）使代谢率峰值达到140%（从180毫克O₂/千克·小时升至252毫克O₂/千克·小时），2.5小时后恢复到常规代谢率。水温以每分钟0.1℃的速度升高至30℃，从升温开始2.5小时后代谢率峰值达到200%。水产养殖中常用麻醉剂（深度麻醉浓度的MS-222、2-苯氧乙醇和丁香油）在麻醉恢复后的最初几分钟以及随后4小时内都会影响代谢率。可以得出结论，代谢率是金鱼对涉及能量消耗和应激的水产养殖操作反应的良好指标。因此，间歇式呼吸测量法是了解和改善水产养殖中鱼类福利的一种有价值的非侵入性工具。
