Leps Anna Sophia, Klein Babette, Schneider Marianne, Goericke-Pesch Sandra
Unit for Reproductive Medicine-Clinic for Small Animals, University of Veterinary Medicine Hannover, 30559 Hannover, Germany.
Laboklin GmbH & Co. KG, 97688 Bad Kissingen, Germany.
Antibiotics (Basel). 2024 Oct 9;13(10):946. doi: 10.3390/antibiotics13100946.
Antimicrobial stewardship is one of the cornerstones in the battle against antimicrobial resistance. Restrictive legislation aims to foster antimicrobial stewardship. Prophylactic prescription of antimicrobials is still a widespread practice in canine breeding management to prevent suspected infectious infertility. The aim of this study was to evaluate the efficacy of restrictive legislation in Germany (Veterinary Home Pharmacy Ordinance, TÄHAV) based on resistance profiles of common bacterial isolates from the vaginal tract by comparing the resistance situation before (time frame (TF1)) and after (TF2) its amendment. In total, results of 13,373 antimicrobial susceptibility tests of bacterial isolates of (n = 5209), beta-hemolytic streptococci (n = 4010), and group (n = 4154) derived from canine vaginal swabs were assessed. Antimicrobial susceptibility testing was performed on pure cultures using the broth microdilution method. Susceptibility to selected antimicrobials was evaluated. Susceptibility of generally increased within TF2 with, however, a significant increase in the number of non-susceptible isolates to cefalexin ( < 0.0001). Beta-hemolytic streptococci exhibited good susceptibility to most antimicrobials. Susceptibility developed ambivalently within the group. Despite an overall positive effect of increased susceptibility, an increase in non-susceptibility to single antimicrobials was detected, possibly indicating a need for refinement of the legislation.
抗菌药物管理是对抗抗菌药物耐药性斗争的基石之一。限制性立法旨在促进抗菌药物管理。在犬类繁殖管理中，预防性使用抗菌药物仍是一种普遍做法，以预防疑似感染性不育。本研究的目的是通过比较德国《兽医家庭药房条例》（TÄHAV）修订前（时间框架（TF1））和修订后（TF2）阴道常见细菌分离株的耐药谱，评估该条例的效果。总共评估了来自犬阴道拭子的13373份细菌分离株的抗菌药敏试验结果，其中包括大肠杆菌（n = 5209）、β-溶血性链球菌（n = 4010）和葡萄球菌属（n = 4154）。使用肉汤微量稀释法对纯培养物进行抗菌药敏试验。评估对选定抗菌药物的敏感性。在TF2期间，大肠杆菌的敏感性总体上有所增加，然而，对头孢氨苄不敏感的分离株数量显著增加（P < 0.0001）。β-溶血性链球菌对大多数抗菌药物表现出良好的敏感性。葡萄球菌属的敏感性变化不定。尽管敏感性增加总体上有积极影响，但检测到对单一抗菌药物不敏感性增加，这可能表明需要完善该立法。
