Wang Xifeng, Tian Xiangru, Liu Zhi, Liu Zhihua, Shang Shuying, Li Haifeng, Qu Jianhang, Chen Pengxiao
School of Biological Engineering, Henan University of Technology, Zhengzhou 450001, China.
School of International Education, Henan University of Technology, Zhengzhou 450001, China.
Insects. 2024 Sep 24;15(10):734. doi: 10.3390/insects15100734.
Corn straw is considered a renewable biomass energy source, and its unreasonable disposal leads to resource waste and environmental pollution. Black soldier fly ( L.) larvae (BSFL) facilitate the bioconversion of various types of organic wastes. In this study, we found that 88% of BSFL survived, and 37.4% of corn straw was digested after 14 days of feeding with corn straw. Contrary to expectations, the pretreatment of corn straw with alkaline hydrogen peroxide did not promote its digestion but rather reduced the growth and survival rates of BSFL. , , and unclassified were the abundant genera in the BSFL gut fed with corn straw. Compared with the standard diet, the relative abundances of carbohydrate metabolism genes, such as the gene abundances of β-glucosidase and α-glucosidase, were higher with corn straw as the substrate. These results suggested that the gut microbial community could regulate suitable and functional microorganisms in response to the substrates. Furthermore, four cellulase-producing strains, namely , , , and , were isolated from the guts of corn straw BSFL. These four strains helped increase the conversion rates of corn straw, the weights of BSFL, and survival rates. In summary, we reared BSFL with corn straw and discovered the functions of gut microorganisms in adapting to the substrates. We also isolated four cellulase-producing strains from the BSFL guts and declared the benefits of BSFL digesting corn straw.
玉米秸秆被认为是一种可再生的生物质能源，其不合理处置会导致资源浪费和环境污染。黑水虻（Hermetia illucens L.）幼虫有助于各类有机废弃物的生物转化。在本研究中，我们发现用玉米秸秆喂养14天后，88%的黑水虻幼虫存活，37.4%的玉米秸秆被消化。与预期相反，用碱性过氧化氢对玉米秸秆进行预处理并未促进其消化，反而降低了黑水虻幼虫的生长和存活率。在以玉米秸秆为食的黑水虻幼虫肠道中，乳杆菌属（Lactobacillus）、拟杆菌属（Bacteroides）和未分类菌属是优势菌属。与标准日粮相比，以玉米秸秆为底物时，碳水化合物代谢基因的相对丰度更高，如β - 葡萄糖苷酶和α - 葡萄糖苷酶的基因丰度。这些结果表明，肠道微生物群落可根据底物调节适宜且具功能的微生物。此外，从以玉米秸秆为食的黑水虻幼虫肠道中分离出了四株产纤维素酶的菌株，即解纤维素金黄杆菌（Chryseobacterium cellulolyticum）、嗜麦芽窄食单胞菌（Stenotrophomonas maltophilia）、纤维弧菌属（Cellulosimicrobium cellulans）和纤维素分解糖多孢菌（Saccharopolyspora cellulolyticus）。这四株菌株有助于提高玉米秸秆的转化率、黑水虻幼虫的体重和存活率。总之，我们用玉米秸秆饲养黑水虻幼虫，发现了肠道微生物在适应底物方面的功能。我们还从黑水虻幼虫肠道中分离出四株产纤维素酶的菌株，并阐述了黑水虻幼虫消化玉米秸秆的益处。
