Liu Xiangjie, Cao Minhui, Mei Wenjuan, Wang Xingliang, Wu Yidong
Sanya Institute of Nanjing Agricultural University, Sanya 572025, China.
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Insects. 2024 Oct 8;15(10):777. doi: 10.3390/insects15100777.
is one of the most serious lepidopteran pests of global importance. With the intensive use of insecticides, has evolved resistance to many insecticides, including the sodium channel blocker insecticides (SCBIs) indoxacarb and metaflumizone. In this study, we investigated the role of the V1848I mutation in the voltage-gated sodium channel (VGSC) in SCBI resistance and its inheritance patterns in through the development and characterization of a near-isogenic resistant strain. The AQ-23 strain of , collected in 2023 from Anqing, Anhui province of China, shows 165-fold resistance to indoxacarb compared with the susceptible WH-S strain. A frequency of 44.6% for the V1848I mutation was detected in the of the AQ-23 strain, while no F1845Y mutation was found. Through repeated backcrossing and marker-assisted selection, the V1848I mutation in the AQ-23 strain was introgressed into the susceptible WH-S strain, creating a near-isogenic strain named WH-1848I. This WH-1848I strain exhibits high levels of resistance to indoxacarb (146-fold) and metaflumizone (431-fold) but remains susceptible to broflanilide and spinosad compared with the WH-S strain. Inheritance analysis revealed that SCBI resistance in the WH-1848I strain is autosomal, nonrecessive, and genetically linked to the V1848I mutation. These findings establish a clear link between the V1848I mutation and SCBI resistance in , offering valuable insights for developing molecular detection tools and resistance management strategies.
是全球最重要的鳞翅目害虫之一。随着杀虫剂的大量使用，其已对包括钠通道阻断剂杀虫剂（SCBIs）茚虫威和甲氧虫酰肼在内的多种杀虫剂产生了抗性。在本研究中，我们通过构建和鉴定一个近等基因抗性品系，研究了电压门控钠通道（VGSC）中的V1848I突变在对SCBIs抗性中的作用及其遗传模式。2023年从中国安徽省安庆市采集的该害虫AQ - 23品系，与敏感的WH - S品系相比，对茚虫威表现出165倍的抗性。在AQ - 23品系的该害虫中检测到V1848I突变的频率为44.6%，而未发现F1845Y突变。通过反复回交和标记辅助选择，将AQ - 23品系中的V1848I突变导入敏感的WH - S品系，创建了一个名为WH - 1848I的近等基因品系。与WH - S品系相比，该WH - 1848I品系对茚虫威（146倍）和甲氧虫酰肼（431倍）表现出高水平抗性，但对溴虫氟苯双酰胺和多杀霉素仍敏感。遗传分析表明，WH - 1848I品系对SCBIs的抗性是常染色体遗传、非隐性的，并且与V1848I突变存在遗传连锁。这些发现明确了V1848I突变与该害虫对SCBIs抗性之间的联系，为开发分子检测工具和抗性管理策略提供了有价值的见解。
