Kim S, Vollberg T M, Ro J Y, Kim M, Sirover M A
Mutat Res. 1986 Feb;173(2):141-5. doi: 10.1016/0165-7992(86)90092-8.
The regulation of the O6-methylguanine methyltransferase was examined during cell proliferation in hypermutable Bloom's syndrome fibroblasts and normal human skin fibroblasts. During synchronous growth following serum stimulation normal human cells enhanced methyltransferase activity 2.4-fold in the absence of exogenous damage as a normal regulatory event during the cell cycle. Methyltransferase activity was increased prior to the induction of DNA replication and of DNA polymerase and was diminished when each replicative activity was maximal. In contrast, although methyltransferase levels in quiescent cells are equivalent, hypermutable Bloom's syndrome cells did not increase methyltransferase at any interval in the cell cycle.
在高度易变的布卢姆综合征成纤维细胞和正常人皮肤成纤维细胞的细胞增殖过程中，对O6-甲基鸟嘌呤甲基转移酶的调节进行了研究。在血清刺激后的同步生长过程中，正常人细胞在没有外源性损伤的情况下，作为细胞周期中的正常调节事件，甲基转移酶活性增强了2.4倍。甲基转移酶活性在DNA复制和DNA聚合酶诱导之前增加，而在每种复制活性达到最大值时降低。相比之下，尽管静止细胞中的甲基转移酶水平相当，但高度易变的布卢姆综合征细胞在细胞周期的任何阶段都没有增加甲基转移酶。
