Escoto-Vasquez Lilibeth Stephania, Portilla-Robertson Javier, Ramírez-Jarquín Josué Orlando, Jacinto-Alemán Luis Fernando, Alonso-Moctezuma Alejandro, Ramírez-Martínez Carla Monserrat, Chanes-Cuevas Osmar Alejandro, Salgado-Chavarria Fabiola
Oral Medicine and Pathology Department, Postgraduate and Research Division, Dentistry School, National Autonomous University of Mexico, Mexico City 04510, Mexico.
Neurosciences Division, Cellular Physiology Institute, National Autonomous University of Mexico, Mexico City 04510, Mexico.
Dent J (Basel). 2024 Oct 14;12(10):327. doi: 10.3390/dj12100327.
Oral cancer holds the sixth position in malignancies worldwide; 90% correspond to oral squamous cell carcinoma (OSCC). Diverse reports suggest that NTRK genes and their receptors are key oncogenesis regulators to tumor progression in human cancers. To analyze the NTRK and Trk expression and their association with clinicopathological features of OSCC in Mexican patients' samples. We analyzed 95 OSCC cases of pan-trk immunoexpression through a software-assisted method. Gene expression was analyzed by RT-qPCR employing the ΔΔCT method. Kruskal-Wallis and Spearman's correlation tests were performed. Our mean age was 62.4 (±16.9) years. A total of 37 cases were tumors in the lateral border of the tongue. Age was significantly associated with the anatomical site. 42% (40 of 95) cases were pan-trk positive. A total of 21 cases showed intense immunoexpression predominantly in poorly differentiated OSCC, with a significant correlation between immunoexpression and age and gender. Gene expression showed that poorly differentiated cases exhibited higher NTRK2 expression, while well-differentiated cases demonstrated NTRK3 significantly higher expression. Our results suggest that NTRK family expression is present in OSCC, with differential expression related to differentiation degree. Additional information about their activation or mutational status could reinforce their potential as a possible primary or adjuvant treatment target.
口腔癌在全球恶性肿瘤中位居第六；90%为口腔鳞状细胞癌（OSCC）。各种报告表明，神经营养酪氨酸激酶（NTRK）基因及其受体是人类癌症肿瘤进展的关键肿瘤发生调节因子。为了分析墨西哥患者样本中NTRK和Trk的表达及其与OSCC临床病理特征的关系。我们通过软件辅助方法分析了95例OSCC病例的泛Trk免疫表达。采用ΔΔCT法通过逆转录定量聚合酶链反应（RT-qPCR）分析基因表达。进行了克鲁斯卡尔-沃利斯检验和斯皮尔曼相关性检验。我们的平均年龄为62.4（±16.9）岁。共有37例肿瘤位于舌侧缘。年龄与解剖部位显著相关。42%（95例中的40例）病例为泛Trk阳性。共有21例主要在低分化OSCC中表现出强烈免疫表达，免疫表达与年龄和性别之间存在显著相关性。基因表达表明，低分化病例表现出较高的NTRK2表达，而高分化病例表现出NTRK3显著较高的表达。我们的结果表明，NTRK家族表达存在于OSCC中，其差异表达与分化程度有关。关于它们的激活或突变状态的更多信息可能会加强它们作为可能的主要或辅助治疗靶点的潜力。
