Quiroz-Castañeda Rosa Estela, Aguilar-Díaz Hugo, Coronado-Villanueva Eduardo, Catalán-Ochoa Diego Israel, Amaro-Estrada Itzel
Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (CENID-SAI, INIFAP), Jiutepec 62574, México.
Pathogens. 2024 Sep 28;13(10):845. doi: 10.3390/pathogens13100845.
Diseases of veterinary importance, such as bovine Anaplasmosis, cause significant economic losses. Due to this, the study of various proteins of the causal agent has focused on surface proteins. However, a vaccine for this disease is not yet available. To this end, in this work, moonlighting proteins (MLPs) are presented as an alternative approach for the design of immunogens against .
The proteins of the strain MEX-15-099-01 were analyzed, and its MLPs were identified. Subsequently, four virulence-associated MLP genes were selected and identified using PCR. The proteins were analyzed using a structural homology approach and the collection of B-cell epitopes was predicted for each MLP. Finally, a pair of AmEno peptides were synthesized and the antigenic potential was tested using an iELISA.
Our bioinformatics analysis revealed the potential of AmEno, AmGroEl, AmEF-Tu, and AmDnaK proteins as promising candidates for designing immunogens. The PCR allowed the gene sequence identification in the genome of the strain MEX-15-099-01. Notably, AmEno-derived synthetic peptides showed antigenicity in an ELISA.
Our study has shed light on the potential use of MLPs for immunogen design, demonstrating the antigenic potential of AmEno.
诸如牛无形体病等具有兽医重要性的疾病会造成重大经济损失。因此，对病原体各种蛋白质的研究主要集中在表面蛋白上。然而，目前尚无针对该疾病的疫苗。为此，在本研究中，兼职蛋白（MLP）被提出作为设计抗该疾病免疫原的一种替代方法。
对菌株MEX - 15 - 099 - 01的蛋白质进行分析，并鉴定其兼职蛋白。随后，使用聚合酶链反应（PCR）选择并鉴定了四个与毒力相关的兼职蛋白基因。采用结构同源性方法对这些蛋白质进行分析，并预测每个兼职蛋白的B细胞表位。最后，合成了一对AmEno肽，并使用间接酶联免疫吸附测定（iELISA）检测其抗原潜力。
我们的生物信息学分析表明，AmEno、AmGroEl、AmEF - Tu和AmDnaK蛋白有潜力成为设计免疫原的理想候选物。PCR技术实现了对菌株MEX - 15 - 099 - 01基因组中基因序列的鉴定。值得注意的是，源自AmEno的合成肽在酶联免疫吸附测定中显示出抗原性。
我们的研究揭示了兼职蛋白在免疫原设计中的潜在用途，证明了AmEno的抗原潜力。
