Aljuwayd Mohammed, Olson Elena G, Abbasi Asim Zahoor, Rothrock Michael J, Ricke Steven C, Kwon Young Min
Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR 72701, USA.
College of Medical Applied Sciences, The Northern Border University, Arar 91431, Saudi Arabia.
Pathogens. 2024 Oct 14;13(10):899. doi: 10.3390/pathogens13100899.
There is an increasing need to develop alternative antimicrobials to replace currently used antibiotics. Phytochemicals, such as essential oils, have garnered significant attention in recent years as potential antimicrobials. However, the mechanisms underlying their bactericidal activities are not yet fully understood. In this study, we investigated the bactericidal activity of eugenol oil against enterica serovar Typhimurium (. Typhimurium) to elucidate its mechanism of action. We hypothesized that eugenol exerts its bactericidal effects through the production of reactive oxygen species (ROS), which ultimately leads to cell death. The result of this study demonstrated that the bactericidal activity of eugenol against . Typhimurium was significantly ( < 0.05) mitigated by thiourea (ROS scavenger) or iron chelator 2,2'-dipyridyl, supporting the hypothesis. This finding contributes to a better understanding of the killing mechanism by eugenol oil.
开发替代抗菌剂以取代目前使用的抗生素的需求日益增长。植物化学物质，如精油，近年来作为潜在的抗菌剂受到了广泛关注。然而，其杀菌活性的潜在机制尚未完全了解。在本研究中，我们研究了丁香油对鼠伤寒沙门氏菌（鼠伤寒血清型）的杀菌活性，以阐明其作用机制。我们假设丁香酚通过产生活性氧（ROS）发挥其杀菌作用，最终导致细胞死亡。本研究结果表明，硫脲（ROS清除剂）或铁螯合剂2,2'-联吡啶可显著（P<0.05）减轻丁香酚对鼠伤寒沙门氏菌的杀菌活性，支持了这一假设。这一发现有助于更好地理解丁香油的杀菌机制。
