Jeju Bio Research Center, Korea Institute of Ocean Science and Technology (KIOST), Jeju 63349, Republic of Korea.
Department of Marine Biology, University of Science and Technology, Daejeon 34113, Republic of Korea.
Mar Drugs. 2024 Oct 7;22(10):461. doi: 10.3390/md22100461.
Abalone is a rich source of nutrition, the viscera of which are discarded as by-product during processing. This study explored the biological activities of peptides derived from abalone viscera (AV). Trypsin-hydrolysate of AV (TAV) was purified into three fractions using a Sephadex G-10 column. Nine bioactive peptides (VAR, NYER, LGPY, VTPGLQY, QFPVGR, LGEW, QLQFPVGR, LDW, and NLGEW) derived from TAV-F2 were sequenced. LGPY, VTPGLQY, LGEW, LDW, and NLGEW exhibited antioxidant properties, with IC values of 0.213, 0.297, 0.289, 0.363, and 0.303 mg/mL, respectively. In vitro analysis determined that the peptides VAR, NYER, VTPGLQY, QFPVGR, LGEW, QLQFPVGR, and NLGEW inhibited ACE, with IC values of 0.104, 0.107, 0.023, 0.023, 0.165, 0.004, and 0.146 mg/mL, respectively. The binding interactions of ACE-bioactive peptide complexes were investigated using docking analysis with the ZDCOK server. VTPGLQT interacted with HIS513 and TYR523, and QLQFPVGR interacted with HIS353, ALA354, GLU384, HIS513, and TYR523, contributing to the inhibition of ACE activity. They also interacted with amino acids that contribute to stability by binding to zinc ions. QFPVGR may form complexes with ACE surface sites, suggesting indirect inhibition. These results indicate that AV is a potential source of bioactive peptides with dual antioxidant and anti-hypertensive dual effects.
鲍鱼是一种营养丰富的食物，其内脏在加工过程中通常被当作副产品丢弃。本研究探索了鲍鱼内脏（AV）衍生肽的生物活性。使用 Sephadex G-10 柱对 AV 的胰蛋白酶水解物（TAV）进行了纯化，得到三个馏分。从 TAV-F2 中分离出 9 种生物活性肽（VAR、NYER、LGPY、VTPGLQY、QFPVGR、LGEW、QLQFPVGR、LDW 和 NLGEW），并对其进行了测序。LGPY、VTPGLQY、LGEW、LDW 和 NLGEW 表现出抗氧化活性，IC 值分别为 0.213、0.297、0.289、0.363 和 0.303 mg/mL。体外分析表明，肽 VAR、NYER、VTPGLQY、QFPVGR、LGEW、QLQFPVGR 和 NLGEW 抑制 ACE 的 IC 值分别为 0.104、0.107、0.023、0.023、0.165、0.004 和 0.146 mg/mL。使用 ZDCOK 服务器的对接分析研究了 ACE-生物活性肽复合物的结合相互作用。VTPGLQT 与 HIS513 和 TYR523 相互作用，而 QLQFPVGR 与 HIS353、ALA354、GLU384、HIS513 和 TYR523 相互作用，这有助于抑制 ACE 活性。它们还与有助于与锌离子结合从而稳定的氨基酸相互作用。QFPVGR 可能与 ACE 表面部位形成复合物，提示间接抑制。这些结果表明，鲍鱼内脏是具有抗氧化和抗高血压双重作用的生物活性肽的潜在来源。
