Gariballa Salah, Al-Bluwi Ghada S M, Yasin Javed
Internal Medicine, College of Medicine & Health Sciences, United Arab Emirates University, Al Ain P.O. Box 15551, United Arab Emirates.
Metabolites. 2024 Sep 27;14(10):524. doi: 10.3390/metabo14100524.
A possible role of vitamin D epimers and metabolites in the measurement and response to treatment of vitamin D has been reported recently. Furthermore, the influence of underlying vitamin D receptor (VDR) genetic polymorphisms which have been linked to diseases such as obesity remains unclear. We therefore aimed to examine the influence of vitamin D3 and calcium supplements on vitamin D epimer and metabolite concentrations in subjects with and those without vitamin D receptor (VDR) gene polymorphisms.
A total of 277 participants who were part of a randomized intervention trial of vitamin D3 and calcium or a placebo for 6 months had clinical and anthropometric assessments. Blood samples were taken for measurements of vitamin D, epimers and metabolites of vitamin D, four vitamin D receptor gene polymorphism SNPs, namely, BsmI, FokI, TaqI, and ApaI, metabolic and inflammatory markers, and related biochemical variables. Repeated-measures analysis of variance was used to assess the between-group difference in cumulative changes in vitamin D epimers and metabolites at 6 months after adjusting for the presence of the 4 VDR genotypes and allele gene polymorphisms.
Overall, 277 participants, with a mean (±SD) age of 41 ± 12 and 204 (74%) of whom were female, were included in the study. We found no statistically significant differences in vitamin D metabolites or (epimers) between male and females or younger subjects compared to those over 40 years of age except in 7C4 BL ( < 0.05). There was a statistically significant difference in 1,25(OH)2D3 concentrations between subjects with and those without genotypes AG and the allele G SNP2_Taql VDR gene polymorphism. Vitamin D3 concentrations were also significantly lower in subjects with the CC SNP3_Apal gene polymorphism compared to those without the CC SNP3 gene. No statistically significant effects were seen on vitamin D epimers and metabolites concentration in response to supplements before or after adjusting for the presence of the 4 VDR genotypes and allele gene polymorphisms.
The CC SNP3 gene had statistically significant influence on vitamin D3 levels. Vitamin D and/or calcium supplements, however, had no effects on vitamin D epimer and metabolite concentration before or after adjusting for the presence of the 4 VDR genotypes and alleles.
近期有报道称维生素D差向异构体和代谢产物在维生素D的测量及治疗反应中可能发挥作用。此外，与肥胖等疾病相关的潜在维生素D受体（VDR）基因多态性的影响仍不明确。因此，我们旨在研究维生素D3和钙补充剂对有和没有维生素D受体（VDR）基因多态性的受试者体内维生素D差向异构体和代谢产物浓度的影响。
共有277名参与者，他们参与了一项为期6个月的维生素D3和钙或安慰剂的随机干预试验，并进行了临床和人体测量评估。采集血样以测量维生素D、维生素D的差向异构体和代谢产物、四个维生素D受体基因多态性单核苷酸多态性（SNP），即BsmI、FokI、TaqI和ApaI、代谢和炎症标志物以及相关生化变量。在调整了4种VDR基因型和等位基因多态性的存在后，采用重复测量方差分析来评估6个月时维生素D差向异构体和代谢产物累积变化的组间差异。
总体而言，277名参与者纳入了研究，平均（±标准差）年龄为41±12岁，其中204名（74%）为女性。我们发现，除了7C4 BL外，男性和女性之间或年轻受试者与40岁以上受试者之间在维生素D代谢产物或（差向异构体）方面没有统计学上的显著差异（<0.05）。有和没有AG基因型以及等位基因G SNP2_Taql VDR基因多态性的受试者之间，1,25(OH)2D3浓度存在统计学上的显著差异。与没有CC SNP3基因的受试者相比，具有CC SNP3_Apal基因多态性的受试者的维生素D3浓度也显著较低。在调整了4种VDR基因型和等位基因多态性的存在前后，补充剂对维生素D差向异构体和代谢产物浓度均未产生统计学上的显著影响。
CC SNP3基因对维生素D3水平有统计学上的显著影响。然而，在调整了4种VDR基因型和等位基因的存在前后，维生素D和/或钙补充剂对维生素D差向异构体和代谢产物浓度均无影响。
