Hulst Rogier van der, Gerretsen Reza R R, Kootker Lisette M, Palstra Sanne W L, Kal Arnoud J, Ammer Saskia T M, Verschoor Shirley P, Borra Lennaert C P, Leeuwen Carina S M van, Verschraagen Miranda, Davies Gareth R, Oostra Roelof-Jan, Touw Daniël J
Netherlands Forensic Institute, The Hague, The Netherlands.
Geology and Geochemistry Cluster, Faculty of Science, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands.
Int J Legal Med. 2025 Jan;139(1):361-374. doi: 10.1007/s00414-024-03357-2. Epub 2024 Oct 25.
Analysis of a single tooth and nail can provide valuable forensic information, including year of birth, year of death, age, sex, DNA-profile, geographic residence during childhood and at time of death and drug exposure. The aim is to minimize the amount of used bodily material and to validate the applicability of a multidisciplinary sampling protocol.
A nail of the big toe, a tooth and blood of seven deceased individuals were collected postmortem. Collected materials were sampled and segmented in accordance with the multidisciplinary sampling protocol. DNA analysis was conducted on the pulp of the tooth, isotope analysis (Sr, Pb, O and C) on the enamel and C-, toxicological and tooth cementum annulation analysis on root segments. DNA-, isotope (Sr, Pb, O and C) -, toxicological-, and C -analysis were conducted on toenail segments. The acquired DNA profiles were compared with profiles acquired from blood.
Material from seven deceased persons was analysed. 45 out of 56 analyses on dental samples were successful, constituting a success rate of 80%. Additionally, 27 out of 35 analyses were successful on nail samples, yielding a success rate of 77%. DNA-, toxicological and C- analyses performed better in nail than in tooth. Isotope analyses performed better in tooth than in nail. A profile with personal characteristics was constructed and matched for 62% of parameters with collected medical information.
The performed sampling protocol for simultaneous multidisciplinary forensic analysis on a single tooth and nail sample provided applicable results and valuable information.
对单个牙齿和指甲进行分析可提供有价值的法医信息，包括出生年份、死亡年份、年龄、性别、DNA图谱、童年时期及死亡时的地理居住信息以及药物暴露情况。目的是尽量减少身体材料的使用量，并验证多学科采样方案的适用性。
在七名死者死后收集其大脚趾指甲、一颗牙齿和血液。按照多学科采样方案对收集到的材料进行采样和分割。对牙髓进行DNA分析，对牙釉质进行同位素分析（锶、铅、氧和碳），对牙根段进行碳、毒理学和牙骨质年轮分析。对脚趾甲段进行DNA、同位素（锶、铅、氧和碳）、毒理学和碳分析。将获得的DNA图谱与从血液中获得的图谱进行比较。
对七名死者的材料进行了分析。对牙齿样本的56次分析中有45次成功，成功率为80%。此外，对指甲样本的35次分析中有27次成功，成功率为77%。DNA、毒理学和碳分析在指甲中的表现优于牙齿。同位素分析在牙齿中的表现优于指甲。构建了具有个人特征的图谱，并与收集到的医疗信息在62%的参数上相匹配。
对单个牙齿和指甲样本进行同步多学科法医分析所执行的采样方案提供了适用的结果和有价值的信息。
