Department of pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran.
Department of pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.
Sci Rep. 2024 Oct 25;14(1):25271. doi: 10.1038/s41598-024-77059-5.
Bovine tuberculosis is one of the most important common infectious diseases between humans and livestock. Cow's milk can be investigated as one of the transmission reservoirs of the disease. Our study was conducted to investigate the presence of Mycobacterium bovis (M. bovis) DNA in cow's milk from different regions of Lorestan province using Touch-down PCR (TD-PCR) method. So, 100 milk samples from industrial and traditional cattle farms were collected and evaluated according to the animal breed, the average age of the animal and the region where the animal was kept. Seven (26.9%) out of the 26 cow's milk samples contaminated with Mycobacterium spp., were diagnosed as M. bovis positive. The cattle with an average age of more than 5 years were most infected with Mycobacterium. Also, the crossbred cattle with an average age of 3 to 5 years, which were kept and raised in tropical areas, showed the highest rate of M. bovis infection. Based on our knowledge, this is the first study regarding the presence of Mycobacterium in cow's milk in Iran.
牛型结核是人类和家畜之间最重要的常见传染病之一。牛奶可以作为该疾病的传播储存库之一进行研究。我们的研究旨在使用降落式聚合酶链反应（TD-PCR）方法调查来自洛雷斯坦省不同地区牛奶中是否存在牛分枝杆菌（M. bovis）DNA。因此，收集了 100 份来自工业和传统奶牛场的牛奶样本，并根据动物品种、动物的平均年龄和饲养地区进行了评估。在 26 份受分枝杆菌污染的牛奶样本中，有 7 份（26.9%）被诊断为 M. bovis 阳性。平均年龄超过 5 岁的牛最容易感染分枝杆菌。此外，在热带地区饲养和饲养的平均年龄为 3 至 5 岁的杂交牛，显示出最高的 M. bovis 感染率。根据我们的知识，这是伊朗首次研究牛奶中是否存在分枝杆菌。
