He Yan-Chun, Wang Meng-Qin, Tie Qing-Qing, Huang Xiao-Wen, Liu Yong-Hong, Li Yun-Qiu, Yang Bin
Chinese Academy of Sciences (CAS) Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, CAS, Guangzhou, 510301, China.
University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, P. R. China.
J Antibiot (Tokyo). 2025 Jan;78(1):64-67. doi: 10.1038/s41429-024-00780-w. Epub 2024 Oct 25.
One new compound named sinulariapeptide F (1) together with one known butyrolactone (2) and seven known peptides (3-9) were isolated from the fungus Simplicillium sp. SCSIO 41222. Their structures and absolute configurations were established using HRESIMS, NMR spectroscopy (H, C, HSQC, HMBC) and marfey's method. All of these compounds were assessed their inhibitory activity of acetylcholinesterase (AChE) and pancreatic lipase (PL). Compounds 4 and 6 were selected to test for the inhibitory activity against programmed cell death-1 (PD-1)/ programmed cell death-ligand 1 (PD-L1). The results indicated that compound 4 displayed potent inhibition activity against PD-1/ PD-L1 with an IC value of 0.656 μM. Furthermore, the docking analysis demonstrated the interactions between 4 and proteins, suggesting PD-L1 to be a probable target for compound 4.
从真菌简梗束霉Simplicillium sp. SCSIO 41222中分离出一种名为sinulariapeptide F (1)的新化合物，以及一种已知的丁内酯(2)和七种已知的肽(3 - 9)。通过高分辨电喷雾电离质谱(HRESIMS)、核磁共振波谱(H、C、HSQC、HMBC)和马尔费伊法确定了它们的结构和绝对构型。对所有这些化合物进行了乙酰胆碱酯酶(AChE)和胰脂肪酶(PL)抑制活性的评估。选择化合物4和6测试其对程序性细胞死亡蛋白1(PD - 1)/程序性细胞死亡配体1(PD - L1)的抑制活性。结果表明，化合物4对PD - 1/PD-L1具有较强的抑制活性，IC值为0.656 μM。此外，对接分析表明了化合物4与蛋白质之间的相互作用，提示PD - L1可能是化合物4的作用靶点。
