从内生真菌SPC3中分离得到的单环菌素A和B。

Simplicilones A and B Isolated from the Endophytic Fungus SPC3.

作者信息

Anoumedem Elodie Gisèle M, Mountessou Bel Youssouf G, Kouam Simeon F, Narmani Abolfazl, Surup Frank

机构信息

Department of Chemistry, Higher Teacher Training College, University of Yaoundé I, Yaoundé P.O. Box 47, Cameroon.

Microbial Drugs Department, Helmholtz-Centre for Infection Research (HZI), Inhoffenstr. 7, 38124 Braunschweig, Germany.

出版信息

Antibiotics (Basel). 2020 Oct 29;9(11):753. doi: 10.3390/antibiotics9110753.

Two new tetracyclic polyketides with a spirocenter, simplicilones A () and B () were isolated from the broth-culture of the endophytic fungus (SPC3) in the course of our screening for new bioactive secondary metabolites. This endophytoic fungus is naturally harboured in the fresh bark of the Cameroonian medicinal plant (Engl. and Diels) Chatrou. The planar structures of the simplicilones were elucidated by MS and 1D as well as 2D NMR spectroscopic techniques. The relative configuration was assigned by NOESY experiments in conjunction with coupling constants; subsequently, the absolute configurations were assigned by the modified Mosher's method. The compounds showed weak cytotoxic effects against the cell line KB3.1 (in vitro cytotoxicity (IC) = 25 µg/mL for , 29 µg/mL for ), but were inactive against the tested Gram-positive and Gram-negative bacteria as well as fungi.

在我们对新型生物活性次生代谢产物的筛选过程中，从内生真菌（SPC3）的发酵液中分离出了两种具有螺中心的新型四环聚酮化合物，即简单霉素A（）和B（）。这种内生真菌天然存在于喀麦隆药用植物（Engl.和Diels）Chatrou的新鲜树皮中。通过质谱、一维和二维核磁共振光谱技术阐明了简单霉素的平面结构。通过NOESY实验结合耦合常数确定了相对构型；随后，通过改良的莫舍尔方法确定了绝对构型。这些化合物对KB3.1细胞系显示出较弱的细胞毒性作用（体外细胞毒性（IC）= 对于为25 µg/mL，对于为29 µg/mL），但对测试的革兰氏阳性和革兰氏阴性细菌以及真菌均无活性。