• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

RNF2通过调节CBX7的泛素化和降解促进软骨肉瘤进展。

RNF2 promotes chondrosarcoma progression by regulating ubiquitination and degradation of CBX7.

作者信息

Wu Yue, Huang Zheng, Luo Ping, Xiang Zhong, Zhang Meng, Chen Zhiwu, Zhou Yalu, Li Jiameng

机构信息

Department of Orthopedics, Beijing Chaoyang Hospital, No.8 Gongti South Rd, Chaoyang District, Beijing, 100020, China.

Department of Orthopedics, Huazhong University of Science and Technology Union Shenzhen Hospital, No. 89 Taoyuan Road, Nanshan District, Shenzhen City, 210009, Guangdong, China.

出版信息

Cancer Metab. 2024 Oct 25;12(1):30. doi: 10.1186/s40170-024-00359-x.

DOI:10.1186/s40170-024-00359-x
PMID:39456039
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11520121/
Abstract

OBJECTIVE

Chondrosarcoma (CHS) is resistant to conventional chemotherapy and radiotherapy and currently lacks effective treatment options when in advanced stages. Accordingly, this research investigated the mechanism of RNF2/CBX7 in CHS to drive the development of molecularly targeted drugs for CHS.

METHODS

RNF2 and CBX7 levels were detected in CHS cells and tissues. RNF2 and CBX7 expression was modulated through cell transfection to examine their effects on cell proliferation, apoptosis, migration, and angiogenesis. The correlation between RNF2 and CBX7 levels was determined, and the ubiquitination level of CBX7 was tested. Protein synthesis was blocked in RNF2-knockdown/overexpressing cells with CHX to assess the effect of RNF2 on CBX7 stability. JJ012 cells transfected with LV-sh-RNF2 were subcutaneously injected into nu/nu nude mice to ascertain the action of RNF2 in the growth and metastasis of CHS.

RESULTS

RNF2 was highly expressed in CHS cells and tissues. RNF2 knockdown curbed CHS cell proliferation, migration, and angiogenesis while promoting apoptosis. RNF2 knockdown in JJ012 cells upregulated CBX7 protein levels and reduced CBX7 ubiquitination, whilst RNF2 had no effect on CBX7 mRNA expression. CBX7 knockdown partially nullified the repressing effects of RNF2 knockdown on CHS cell proliferation, migration, and angiogenesis, and CBX7 overexpression partially abolished the promotional effects of RNF2 overexpression. LV-sh-RNF2 prominently restricted tumor growth and weight and declined lung metastatic nodules and Ki-67-positive cells in mice.

CONCLUSION

RNF2 fosters CHS progression by elevating CBX7 degradation via the ubiquitination pathway.

摘要

目的

软骨肉瘤（CHS）对传统化疗和放疗具有抗性，在晚期时目前缺乏有效的治疗选择。因此，本研究调查了RNF2/CBX7在CHS中的作用机制，以推动CHS分子靶向药物的开发。

方法

检测CHS细胞和组织中RNF2和CBX7的水平。通过细胞转染调节RNF2和CBX7的表达，以检查它们对细胞增殖、凋亡、迁移和血管生成的影响。确定RNF2和CBX7水平之间的相关性，并检测CBX7的泛素化水平。用环己酰亚胺（CHX）阻断RNF2敲低/过表达细胞中的蛋白质合成，以评估RNF2对CBX7稳定性的影响。将转染了LV-sh-RNF2的JJ012细胞皮下注射到裸鼠体内，以确定RNF2在CHS生长和转移中的作用。

结果

RNF2在CHS细胞和组织中高表达。RNF2敲低抑制了CHS细胞的增殖、迁移和血管生成，同时促进了细胞凋亡。JJ012细胞中RNF2敲低上调了CBX7蛋白水平并降低了CBX7的泛素化，而RNF2对CBX7 mRNA表达没有影响。CBX7敲低部分抵消了RNF2敲低对CHS细胞增殖、迁移和血管生成的抑制作用，而CBX7过表达部分消除了RNF2过表达的促进作用。LV-sh-RNF2显著抑制了小鼠的肿瘤生长和重量，并减少了肺转移结节和Ki-67阳性细胞。

结论

RNF2通过泛素化途径提高CBX7的降解，从而促进CHS进展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7324/11520121/9f12bd287414/40170_2024_359_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7324/11520121/5ad10d7cd747/40170_2024_359_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7324/11520121/9a14d682a8ce/40170_2024_359_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7324/11520121/f8e5df9d5312/40170_2024_359_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7324/11520121/421701525bb2/40170_2024_359_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7324/11520121/7e065debeabc/40170_2024_359_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7324/11520121/1147c9b9e746/40170_2024_359_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7324/11520121/9f12bd287414/40170_2024_359_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7324/11520121/5ad10d7cd747/40170_2024_359_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7324/11520121/9a14d682a8ce/40170_2024_359_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7324/11520121/f8e5df9d5312/40170_2024_359_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7324/11520121/421701525bb2/40170_2024_359_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7324/11520121/7e065debeabc/40170_2024_359_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7324/11520121/1147c9b9e746/40170_2024_359_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7324/11520121/9f12bd287414/40170_2024_359_Fig5_HTML.jpg

相似文献

1
RNF2 promotes chondrosarcoma progression by regulating ubiquitination and degradation of CBX7.RNF2通过调节CBX7的泛素化和降解促进软骨肉瘤进展。
Cancer Metab. 2024 Oct 25;12(1):30. doi: 10.1186/s40170-024-00359-x.
2
Knockdown of RNF2 induces apoptosis by regulating MDM2 and p53 stability.敲低 RNF2 通过调节 MDM2 和 p53 的稳定性诱导细胞凋亡。
Oncogene. 2014 Jan 23;33(4):421-8. doi: 10.1038/onc.2012.605. Epub 2013 Jan 14.
3
RNF2 promotes the progression of colon cancer by regulating ubiquitination and degradation of IRF4.RNF2 通过调节 IRF4 的泛素化和降解促进结肠癌的进展。
Biochim Biophys Acta Mol Cell Res. 2022 Jan;1869(1):119162. doi: 10.1016/j.bbamcr.2021.119162. Epub 2021 Oct 17.
4
Ring finger protein 2 promotes colorectal cancer progression by suppressing early growth response 1.环指蛋白 2 通过抑制早期生长反应 1 促进结直肠癌的进展。
Aging (Albany NY). 2020 Dec 19;12(24):26199-26220. doi: 10.18632/aging.202396.
5
Knockdown of RNF2 induces cell cycle arrest and apoptosis in prostate cancer cells through the upregulation of TXNIP.RNF2基因敲低通过上调TXNIP诱导前列腺癌细胞的细胞周期阻滞和凋亡。
Oncotarget. 2017 Jan 17;8(3):5323-5338. doi: 10.18632/oncotarget.14142.
6
Circ_0004104 Accelerates the Progression of Gastric Cancer by Regulating the miR-539-3p/RNF2 Axis.环状 RNA 0004104 通过调控 miR-539-3p/RNF2 轴促进胃癌的进展。
Dig Dis Sci. 2021 Dec;66(12):4290-4301. doi: 10.1007/s10620-020-06802-5. Epub 2021 Jan 15.
7
Radiosensitization of esophageal carcinoma cells by knockdown of RNF2 expression.通过敲低RNF2表达对食管癌细胞进行放射增敏
Int J Oncol. 2016 May;48(5):1985-96. doi: 10.3892/ijo.2016.3404. Epub 2016 Feb 19.
8
Oncogenic role of the chromobox protein CBX7 in gastric cancer.CBX7 染色盒蛋白在胃癌中的致癌作用。
J Exp Clin Cancer Res. 2010 Aug 19;29(1):114. doi: 10.1186/1756-9966-29-114.
9
E3 ligase RNF2 inhibits porcine circovirus type 3 replication by targeting its capsid protein for ubiquitination-dependent degradation.E3 连接酶 RNF2 通过靶向其衣壳蛋白进行泛素化依赖降解来抑制猪圆环病毒 3 的复制。
J Virol. 2024 Aug 20;98(8):e0022324. doi: 10.1128/jvi.00223-24. Epub 2024 Jul 24.
10
[Effect of RNF2 knockdown on apoptosis and radiosensitivity in glioma U87 cells].[RNF2基因敲低对胶质瘤U87细胞凋亡及放射敏感性的影响]
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2014 May;30(5):471-5.

引用本文的文献

1
Unraveling the molecular mechanisms of paclitaxel in high-grade serous ovarian cancer through network pharmacology.通过网络药理学揭示紫杉醇在高级别浆液性卵巢癌中的分子机制。
Sci Rep. 2025 May 12;15(1):16445. doi: 10.1038/s41598-025-00658-3.

本文引用的文献

1
CBX4 deletion promotes tumorigenesis under Kras background by inducing genomic instability.CBX4 缺失通过诱导基因组不稳定性在 Kras 背景下促进肿瘤发生。
Signal Transduct Target Ther. 2023 Sep 12;8(1):343. doi: 10.1038/s41392-023-01623-0.
2
Chondrosarcoma: A Clinical Review.软骨肉瘤：临床综述
J Clin Med. 2023 Mar 26;12(7):2506. doi: 10.3390/jcm12072506.
3
Targeted protein degradation in cancers: Orthodox PROTACs and beyond.癌症中的靶向蛋白质降解：传统的PROTACs及其他。
Innovation (Camb). 2023 Mar 15;4(3):100413. doi: 10.1016/j.xinn.2023.100413. eCollection 2023 May 15.
4
Association of FBXW11 levels with tumor development and prognosis in chondrosarcoma.FBXW11 水平与软骨肉瘤肿瘤发生和预后的相关性研究。
Cancer Biomark. 2022;35(4):429-437. doi: 10.3233/CBM-210426.
5
Pan-cancer analysis of Chromobox (CBX) genes for prognostic significance and cancer classification.泛癌症分析 Chromobox (CBX) 基因在预后意义和癌症分类中的作用。
Biochim Biophys Acta Mol Basis Dis. 2023 Jan 1;1869(1):166561. doi: 10.1016/j.bbadis.2022.166561. Epub 2022 Sep 29.
6
Chromobox Homologue 7 Acts as a Tumor Suppressor in Both Lung Adenocarcinoma and Lung Squamous Cell Carcinoma via Inhibiting ERK/MAPK Signaling Pathway.染色体盒同源物7通过抑制ERK/MAPK信号通路在肺腺癌和肺鳞状细胞癌中发挥肿瘤抑制作用。
Evid Based Complement Alternat Med. 2022 May 19;2022:4952185. doi: 10.1155/2022/4952185. eCollection 2022.
7
The role of ubiquitination and deubiquitination in tumor invasion and metastasis.泛素化和去泛素化在肿瘤侵袭和转移中的作用。
Int J Biol Sci. 2022 Mar 6;18(6):2292-2303. doi: 10.7150/ijbs.69411. eCollection 2022.
8
The Next Frontier: Translational Development of Ubiquitination, SUMOylation, and NEDDylation in Cancer.下一个前沿：泛素化、SUMO 化和 NEDDylation 在癌症中的转化发展。
Int J Mol Sci. 2022 Mar 23;23(7):3480. doi: 10.3390/ijms23073480.
9
BMI1 promotes osteosarcoma proliferation and metastasis by repressing the transcription of SIK1.BMI1通过抑制SIK1的转录促进骨肉瘤的增殖和转移。
Cancer Cell Int. 2022 Mar 27;22(1):136. doi: 10.1186/s12935-022-02552-8.
10
Therapeutic Targets and Emerging Treatments in Advanced Chondrosarcoma.晚期软骨肉瘤的治疗靶点和新兴治疗方法。
Int J Mol Sci. 2022 Jan 20;23(3):1096. doi: 10.3390/ijms23031096.