Scalcon Valeria, Fiorese Federico, Albanesi Marica, Folda Alessandra, Betti Gianfranco, Bellamio Marco, Feller Emiliano, Lodovichi Claudia, Arrigoni Giorgio, Marin Oriano, Rigobello Maria Pia
Department of Biomedical Sciences, University of Padova, Via Ugo Bassi 58/b, 35132 Padova, Italy.
Padova Neuroscience Center (PNC), University of Padova, Via Orus 2, 35129 Padova, Italy.
Antioxidants (Basel). 2024 Oct 10;13(10):1221. doi: 10.3390/antiox13101221.
The discarding of agri-food by-products is a stringent problem due to their high environmental impact. Recovery strategies can lead to a reduction of waste and result in new applications. Agri-food waste represents a source of bioactive molecules, which could promote health benefits. The primary goal of this research has been the assessment of the antioxidant activity of milk permeate, a dairy farm by-product, and the isolation and identification of peptide fractions endowed with antioxidant activity. The chromatographic extraction of the peptide fractions was carried out, and the peptides were identified by mass spectrometry. The fractions showed radical scavenging activity in vitro. Moreover, the results in the Caco-2 cell model demonstrated that the peptide fractions were able to protect from oxidative stress by stimulating the Keap1/Nrf2 antioxidant signaling pathway, increasing the transcription of antioxidant enzymes. In addition, the bioactive peptides can affect cellular metabolism, increasing mitochondrial respiration. The action of the peptide fractions was also assessed in vivo on a zebrafish model and resulted in the protection of the whole organism from the adverse effects of acute cold stress, highlighting their strong capability to protect from an oxidative insult. Altogether, the results unveil novel recovery strategies for food by-products as sources of antioxidant bioactive peptides that might be utilized for the development of functional foods.
由于农业食品副产品对环境影响大，其丢弃是一个严峻的问题。回收策略可以减少浪费并带来新的应用。农业食品废弃物是生物活性分子的一个来源，这些生物活性分子可能对健康有益。本研究的主要目标是评估乳清渗透液（一种奶牛场副产品）的抗氧化活性，以及分离和鉴定具有抗氧化活性的肽段。对肽段进行了色谱提取，并通过质谱对肽进行了鉴定。这些肽段在体外表现出自由基清除活性。此外，在Caco-2细胞模型中的结果表明，这些肽段能够通过刺激Keap1/Nrf2抗氧化信号通路来保护细胞免受氧化应激，增加抗氧化酶的转录。此外，生物活性肽可以影响细胞代谢，增加线粒体呼吸。还在斑马鱼模型上对肽段的作用进行了体内评估，结果表明其能保护整个生物体免受急性冷应激的不利影响，突出了它们抵御氧化损伤的强大能力。总之，这些结果揭示了将食品副产品作为抗氧化生物活性肽来源的新型回收策略，这些策略可用于开发功能性食品。
