College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, China.
Drug Discovery Research Center, Southwest Medical University, Luzhou 646099, China.
Genes (Basel). 2024 Sep 27;15(10):1261. doi: 10.3390/genes15101261.
The gene-encoding farnesyl diphosphate O-methyltransferase 6 (FAMeT 6) is a member of the farnesyl diphosphate O-methyltransferase family. Our previous studies have demonstrated its influence on juvenile hormone levels in third instar silkworm larvae. we utilized transcriptomic and metabolomic techniques to investigate the changes in third instar larvae at 0, 12, and 24 h following overexpression. (1) The differentially expressed homologous genes were enriched in detoxification-related pathways at all three time points. (2) Transcription factor analysis of DEGs indicated a predominant presence of ZF-C2H2. (3) The metabolite-related network suggested that may influence the metabolism of silkworm larvae through the ABC transporters, purine metabolism, and tyrosine metabolism pathways. (4) The differential gene count, differential metabolite count, and types of metabolites at the three time points indicated a shift in the regulatory focus within the larvae as time progresses, with the inflection point of regulation occurring at the third instar larval stage, 12 h. In summary, our research indicates that the regulatory role of occurs within the context of the domestic silkworm's own growth and development regulation.
法呢基二磷酸 O-甲基转移酶 6（FAMeT6）基因编码的蛋白质是法呢基二磷酸 O-甲基转移酶家族的成员。我们之前的研究表明，它影响三龄家蚕幼虫的保幼激素水平。我们利用转录组学和代谢组学技术研究了 0、12 和 24 h 后过表达时三龄幼虫的变化。（1）在所有三个时间点，差异表达的同源基因富集在解毒相关途径中。（2）DEGs 的转录因子分析表明，ZF-C2H2 占主导地位。（3）代谢物相关网络表明，可能通过 ABC 转运蛋白、嘌呤代谢和酪氨酸代谢途径影响家蚕幼虫的代谢。（4）三个时间点的差异基因计数、差异代谢物计数和代谢物类型表明，随着时间的推移，幼虫的调节焦点发生了变化，调节的转折点发生在三龄幼虫阶段，12 h。总之，我们的研究表明，在家蚕自身生长发育调控的背景下，发挥着调节作用。
