Zhang Boyu, Hao Yajuan, Liu Haiyan, Wu Jiarun, Lu Lu, Wang Xinfeng, Bajpai Akhilesh K, Yang Xi
Department of Hematology, Affiliated Hospital of Nantong University, Nantong 226007, China.
Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China.
Biomedicines. 2024 Sep 27;12(10):2211. doi: 10.3390/biomedicines12102211.
: N-methyladenosine (mA), is the most common modification found in mRNA and lncRNA in higher organisms and plays an important role in physiology and pathology. However, its role in pan-cancer has not been explored. : A total of 31 mA modification regulators, including 12 writers, 2 erasers, and 17 readers are identified in the current study. The functional analysis of the regulators results in the enrichment of processes, primarily related to RNA modification and metabolism, and the PPI network reveals multiple interactions among the regulators. The mRNA expression analysis reveals a high expression for most of the regulators in pan-cancer. Most of the mA regulators are found to be mutated across the cancers, with , and having a higher frequency rate. Significant correlations of the regulators with clinicopathological parameters, such as age, gender, tumor stage, and grade are identified in pan-cancer. The mA regulators' expression is found to have significant positive correlations with the miRNAs in pan-cancer. The expression pattern of the mA regulators is able to classify the tumors into different subclusters as well as into high- and low-risk groups. These tumor groups show differential patterns in terms of their immune cell infiltration, tumor stemness score, genomic heterogeneity score, expression of immune regulatory/checkpoint genes, and correlations between the regulators and the drugs. : Our study provide a comprehensive overview of the functional roles, genetic and epigenetic alterations, and prognostic value of the RNA mA regulators in pan-cancer.
N-甲基腺苷（mA）是高等生物中mRNA和lncRNA中最常见的修饰，在生理和病理过程中发挥着重要作用。然而，其在泛癌中的作用尚未得到探索。：在本研究中，共鉴定出31种mA修饰调节因子，包括12种写入器、2种擦除器和17种读取器。对这些调节因子的功能分析导致了主要与RNA修饰和代谢相关的过程的富集，蛋白质-蛋白质相互作用网络揭示了调节因子之间的多种相互作用。mRNA表达分析显示，大多数调节因子在泛癌中高表达。发现大多数mA调节因子在各种癌症中发生突变，其中[具体癌症1]、[具体癌症2]和[具体癌症3]的突变频率较高。在泛癌中确定了调节因子与临床病理参数如年龄、性别、肿瘤分期和分级之间的显著相关性。发现mA调节因子的表达与泛癌中的miRNA有显著正相关。mA调节因子的表达模式能够将肿瘤分为不同的亚群以及高风险和低风险组。这些肿瘤组在免疫细胞浸润、肿瘤干性评分、基因组异质性评分、免疫调节/检查点基因的表达以及调节因子与药物之间的相关性方面表现出不同的模式。：我们的研究全面概述了RNA mA调节因子在泛癌中的功能作用、遗传和表观遗传改变以及预后价值。
