线粒体靶向抗氧化剂MitoQ改善淘汰奶牛的体外成熟及后续胚胎发育

Mitochondria-Targeted Antioxidant MitoQ Improves In Vitro Maturation and Subsequent Embryonic Development from Culled Cows.

作者信息

Feng Zhihao, Shi Junsong, Ren Jiajie, Luo Lvhua, Liu Dewu, Guo Yongqing, Sun Baoli, Liu Guangbin, Deng Ming, Li Yaokun

机构信息

College of Animal Science, South China Agricultural University, Guangzhou 510642, China.

Yunfu Sub-Center of Guangdong Laboratory for Lingnan Modern Agriculture, Yunfu 527300, China.

出版信息

Animals (Basel). 2024 Oct 11;14(20):2929. doi: 10.3390/ani14202929.

The purpose of this study was to investigate the effects and mechanisms of MitoQ on the IVM of culled bovine oocytes and subsequent embryonic development. The results revealed that in comparison to the control group (0 µmol/L), the IVM rate ( < 0.05) and subsequent blastocyst rate ( < 0.05) of the low-concentration 1 and 5 µmol/L MitoQ treatment group were increased. The level of ROS ( < 0.05) in the MitoQ treatment group was decreased in comparison to the control group. Additionally, the level of GSH, MMP, ATP, and mt-DNA in the MitoQ treatment group was increased ( < 0.05) in comparison to the control group. The expression level of BAX was decreased ( < 0.05) in the MitoQ treatment group, and the BCL2, DNM1, Mfn2, SOD, and CAT were increased ( < 0.05). In conclusion, MitoQ improved mitochondrial dysfunction, increased mitochondrial activity during IVM, and reduced oxidative stress, resulting in increased IVM rates and subsequent embryonic development from culled cows.

本研究的目的是探讨线粒体辅酶Q（MitoQ）对淘汰奶牛卵母细胞体外成熟（IVM）及随后胚胎发育的影响和机制。结果显示，与对照组（0 μmol/L）相比，低浓度1和5 μmol/L MitoQ处理组的IVM率（<0.05）和随后的囊胚率（<0.05）均有所提高。与对照组相比，MitoQ处理组的活性氧水平（<0.05）降低。此外，与对照组相比，MitoQ处理组的谷胱甘肽（GSH）、线粒体膜电位（MMP）、三磷酸腺苷（ATP）和线粒体DNA（mt-DNA）水平均升高（<0.05）。MitoQ处理组中促凋亡蛋白BAX的表达水平降低（<0.05），而抗凋亡蛋白BCL2、动力相关蛋白1（DNM1）、线粒体融合蛋白2（Mfn2）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的表达水平升高（<0.05）。总之，MitoQ改善了线粒体功能障碍，增加了IVM期间的线粒体活性，并降低了氧化应激，从而提高了淘汰奶牛卵母细胞的IVM率及随后的胚胎发育率。