Department of Traditional Chinese Medicine, Institute of Traditional Chinese Medicine, Capital Medical University, Beijing, China.
Aging (Albany NY). 2021 Oct 14;13(19):23328-23337. doi: 10.18632/aging.203626.
To study the effect of Yu Linzhu on ovarian function and mitochondria in natural aging mice.
Female BALB/c mice were selected as normal group at 7-8 weeks and natural aging group at 9 months. The natural aging group was divided into Yu Linzhu intervention group and non-intervention group by intragastric administration once a day for 6 weeks. The morphology and blood flow of ovary were observed by ultrasound. Ovarian morphology and follicle were observed by HE staining. Hormone levels were analyzed by ELISA. Serum oxidative stress were detected by radioimmunoassay. The distribution of mitochondria in oocytes was observed by fluorescence staining. The ultrastructure of oocytes and the morphology of mitochondria were observed under electron microscope. The mitochondrial membrane potential was detected by JC-1.
Two groups of aging mice had serious disturbance of estrus cycle. The ovarian area of the mice in the aging non-intervention group was smaller than that in the normal group, and the ovarian area of the mice in the aging intervention group recovered. The ovarian blood flow was weak or even disappear in the aging non-intervention group, and the blood flow in the intervention group was improved. The ovarian volume of mice in the non-intervention group was smaller than that in the normal group. Some ovarian tissues were adhered to the surrounding tissues. While in the intervention group, the ovarian volume increased, the degree of adhesion decreased, the infiltration of ovarian interstitial lymphocytes decreased, and the zona pellucida recovered. Granular cell arrangement returned neatly, egg cell shape recover regular and the number also increased. In the non-intervention group, E2 (Estrogen), AMH (Anti-Mullerian hormone) decreased ( = 0.0092 and = 0.0334, respectively), FSH (Follicle stimulating hormone) increased ( < 0.0001). In the intervention group, FSH decreased ( = 0.0002), LH (luteinizing hormone) decreased and E2, AMH increased. In the non-intervention group, GSH-Px (Glutathione peroxidase) decreased ( = 0.0129), SOD (Superoxide dismutase) decreased, ROS (reactive oxidative species), MDA (Malondialdehyde) increased. In the aging intervention group, ROS, MDA decreased and GSH-Px increased. In the non-intervention group, mitochondrial expression was scattered at the concentrated distribution point, the length of mitochondria was mostly long and the average volume increased, the density decreased, the number decreased and some mitochondria fused, and lesions such as swelling, vacuolar degeneration and inclusion body formation, membrane potential decreased ( = 0.0002). In the aging intervention group, mitochondria were evenly distributed, the mitochondria were basically round, the distribution density was moderate, the inner ridge was clear, and the membrane potential of the aging intervention group increased.
Yu Linzhu can improve the ovarian function of natural aging mice by improving the mitochondrial function of oocytes.
研究玉灵珠对自然衰老小鼠卵巢功能及线粒体的影响。
选择 7-8 周龄的雌性 BALB/c 小鼠为正常组，9 月龄为自然衰老组。自然衰老组每天经胃给药 1 次，连续 6 周，分为玉灵珠干预组和非干预组。超声观察卵巢形态及血流。HE 染色观察卵巢形态和卵泡。ELISA 法分析激素水平。放射免疫法检测血清氧化应激。荧光染色观察卵母细胞中线粒体的分布。电镜观察卵母细胞的超微结构和线粒体的形态。JC-1 检测线粒体膜电位。
两组衰老小鼠的动情周期均出现严重紊乱。衰老非干预组小鼠的卵巢面积小于正常组，而衰老干预组的卵巢面积恢复。衰老非干预组的卵巢血流较弱甚至消失，而干预组的血流得到改善。非干预组小鼠的卵巢体积较小，部分卵巢组织与周围组织粘连。而在干预组中，卵巢体积增大，粘连程度降低，卵巢间质淋巴细胞浸润减少，囊泡恢复。颗粒细胞排列整齐，卵母细胞形态恢复正常，数量增加。非干预组中，E2（雌激素）、AMH（抗苗勒管激素）下降（=0.0092 和=0.0334），FSH（卵泡刺激素）升高（<0.0001）。干预组中，FSH 下降（=0.0002），LH（黄体生成素）下降，E2、AMH 升高。非干预组中，GSH-Px（谷胱甘肽过氧化物酶）下降（=0.0129），SOD（超氧化物歧化酶）下降，ROS（活性氧）、MDA（丙二醛）升高。在衰老干预组中，ROS、MDA 下降，GSH-Px 升高。非干预组中线粒体表达呈点状集中分布，线粒体长度多为长，平均体积增大，密度降低，数量减少，部分线粒体融合，出现肿胀、空泡变性和包涵体形成等病变，膜电位下降（=0.0002）。衰老干预组中线粒体均匀分布，线粒体基本呈圆形，分布密度适中，内嵴清晰，膜电位升高。
玉灵珠可通过改善卵母细胞线粒体功能，改善自然衰老小鼠的卵巢功能。
