Dragon Delphine, Jansen Wiebke, Dumont Helene, Wiggers Laetitia, Coupeau Damien, Saulmont Marc, Taminiau Bernard, Muylkens Benoit, Daube Georges
Integrated Veterinary Research Unit, Faculty of Science, Université de Namur, Rue de Bruxelles 61, 5000 Namur, Belgium.
Department of Food Sciences, Microbiology, Fundamental and Applied Research for Animal & Health (FARAH), Faculty of Veterinary Medicine, Université de Liège, Avenue de Cureghem 10, 4000 Liège, Belgium.
Animals (Basel). 2024 Oct 21;14(20):3038. doi: 10.3390/ani14203038.
Major efforts have been made to reduce the use of colistin in livestock since the discovery of the plasmid-borne mobile colistin resistance () gene in a decade ago, to curb the burden of its potential transmission to other bacterial species, spread between animals, humans and the environment. This study explored the longitudinal prevalence and characteristics of colistin-resistant and extended-spectrum beta-lactamase-producing (ESBL) via in vivo fecal and ex vivo carcass swabs from two batches of conventional indoor and organic outdoor Wallon meat sheep from birth to slaughter in 2020 and 2021. Antimicrobial susceptibility testing via broth microdilution revealed that n = 16/109 (15%) isolates from conventional meat lamb fecal samples had a reduced colistin sensitivity (MIC = 0.5 μg/mL) and thereof, n = 9/109 (8%) were multi-drug-resistant , while no resistant isolates were recovered from their carcasses. Sequencing revealed causative genes, indicating that the reduced sensitivity to colistin was not plasmid-borne. While the sample size was small (n = 32), no colistin-resistant and ESBL-producing were isolated from the organic meat sheep and their carcasses, potentially due to the different husbandry conditions. Prudent and judicious antimicrobial use and strict slaughter hygiene remain imperative for effective risk management to protect consumers in a sustainable One Health approach.
自十年前发现质粒介导的可移动黏菌素抗性（）基因以来，人们已做出重大努力减少黏菌素在牲畜中的使用，以遏制其向其他细菌物种潜在传播、在动物、人类和环境之间传播的负担。本研究通过对2020年和2021年两批从出生到屠宰的传统室内养殖和有机户外养殖的瓦隆肉用绵羊进行体内粪便和体外胴体拭子检测，探索了耐黏菌素和产超广谱β-内酰胺酶（ESBL）的纵向流行情况及特征。通过肉汤微量稀释法进行的药敏试验显示，来自传统肉用羔羊粪便样本的n = 16/109（15%）株分离菌对黏菌素的敏感性降低（MIC = 0.5 μg/mL），其中n = 9/109（8%）为多重耐药菌，而从其胴体中未分离出耐药菌株。测序揭示了致病基因，表明对黏菌素敏感性降低并非由质粒介导。虽然样本量较小（n = 32），但从有机肉用绵羊及其胴体中未分离出耐黏菌素和产ESBL的菌株，这可能是由于不同的饲养条件所致。为了以可持续的“同一健康”方法保护消费者，谨慎和明智地使用抗菌药物以及严格的屠宰卫生对于有效的风险管理仍然至关重要。
